The NJ Mass Spectrometry Discussion Group is pleased to announce our September 2019 Annual Vendor Show and Symposium.
NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area.
Date: Tuesday, September 17, 2019
Venue: Somerville Elks Lodge 1068
375 Union Avenue
Bridgewater, NJ 08807
Please register here. Registration is free, compliments of our sponsors.
A limited number of free drink tickets will be provided to early attendees!
The Vendor Show features the following sponsors / vendors / collaborators on site (listed in the order of registration):[raw]
3:00 – 5:00 PM Registration, Vendor Show, and Poster Session
5:00 – 5:45 PM Prof Benjamin A. Garcia (University of Pennsylvania)
5:45 – 7:00 PM Buffet Dinner (raffle prizes will be announced)
7:00 – 8:00 PM Prof Vicki Wysocki (The Ohio State University)
Speaker 1: Prof Benjamin Garcia
University of Pennsylvania
“Quantitative Proteomics for Understanding the Epigenetic Cancer Code”
Abstract for Prof Garcia:
Histones are small proteins that package DNA into chromosomes, and a large number of studies have showed that several post-translational modification (PTM) sites on the histones are associated with both gene activation and silencing. Along with DNA and small non-coding RNA, histone PTMs make up epigenetic mechanisms that control gene expression patterns outside of DNA sequence mutations. Dysregulation of these chromatin networks underlie several human diseases such as cancer. Here I will give an update on technology advancements that have allowed for high-throughput quantitative analyses of histone PTMs and chromatin structure, and how we are applying these methods to understand epigenetic reprogramming found in malignant peripheral nerve sheath tumors (MPNSTs). MPNST is an aggressive sarcoma with recurrent loss of function alterations in polycomb-repressive complex 2 (PRC2), a histone-modifying complex involved in transcriptional silencing.
Speaker 2: Prof Vicki Wysocki
The Ohio State University
“Native Mass Spectrometry and Surface-Induced Dissociation: Characterization of Protein and Nucleoprotein Complexes”
Abstract for Prof Wysocki:
Characterization of the overall topology and inter-subunit contacts of protein complexes, and their assembly/disassembly and unfolding pathways, is critical because protein complexes regulate key biological processes, including processes important in understanding and controlling disease. Tools to address structural biology problems continue to improve. Native mass spectrometry and associated technologies are becoming an increasingly important component of the structural biology toolbox. When the mass spectrometry approach is used early or mid-course in a structural characterization project, it can provide answers quickly using small sample amounts and samples that are not fully purified. Integration of sample preparation/purification with effective dissociation methods, ion mobility, and computational approaches provide a MS workflow that can be enabling in biochemical, synthetic biology, and systems biology approaches. Beyond what MS can provide as a stand-alone tool, MS can also guide and/or be integrated with other structural biology approaches such as NMR, X-ray crystallography, and cryoEM. MS can determine whether the complex of interest exists in a single or in multiple oligomeric states and can provide characterization of topology/intersubunit connectivity, and other structural features. Examples will be presented to illustrate the role MS and surface-induced dissociation can play in guiding a structural biology workflow and will include designed protein complexes and isolated or recombinant protein and nucleoprotein complexes.