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P Jane Gale

The NJ Mass Spectrometry Discussion Group is pleased to announce our June 27, 2023, Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday, June 27, 2023

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908) 707-1545

Sponsor: NJ-MSDG

Please   register here.   It’s free, including dinner, but registration is required.

Masks or no masks are respected and welcomed.


5:30 PM    Social and Registration
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Srinivas Chakravartula

Speaker 1: Srinivas Chakravartula, Ph.D, Mass Spectrometry Facility, Department of Chemistry and Chemical Biology. Rutgers, The State University of NJ
“Qualitative classification of tissue amyloidosis and subtyping by Laser Capture Microdissection (LMD) – LC-Mass spectrometry”

Abstract:  Amyloidosis is a disease characterized by abnormal deposits of insoluble polymeric protein fibrils in tissue and organs. Condition of deposition of amyloid tissue is known as Amyloidosis. In my talk I will demonstrate an approach which simplifies, analysis of these deposits by utilizing high flow liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) of laser micro dissection (LMD) of Congo red +Ve amyloid deposits of formalin fixed paraffin embedded tissues (FFPE) from pathology preparations. The results presented demonstrate the validity of shotgun faster proteomics for precise and accurate identification and classification of amyloid subtypes that is practical for clinical application because of its shorter turnaround time bringing the state of the art pathological diagnosis of amyloidosis into the realm of routine surgical pathology practice to make the contribution of mass spectrometry even more powerful and meaningful for faster patient management decisions by clinicians and also for the effective treatment of amyloidosis. On the other hand, my LC-MS/MS assay is not just restricted for amyloid therapeutics but also opens the possibility of other tissues available to proteomics assays in studying other disease pathologies and for biomarker discoveries.

Dr Gene HallSpeaker 2: Dr Gene Hall, PhD. Professor of Analytical Chemistry Department of Chemistry and Chemical Biology Rutgers, The State University of NJ
“Use of ICP-MS to Determine Sources of Pb in NJ Homeowner’s Tap Water: A Case for Sequential Sampling”

Abstract:  We used an argon ion source to ionize the elements from Li to U in NJ homeowners, daycare centers, and elementary school tap water samples and plumbing materials. The ionized elements were sucked into a single quadrupole mass analyzer to determine concentrations and isotope ratios to determine Pb’s source(s) in the various samples. The combined methods are called inductively coupled plasma mass spectrometry (ICP-MS).

This seminar will focus on using ICP-MS to determine Pb’s source(s) in tap water samples collected from NJ homes, daycare centers, and elementary schools. A simple collect and inject workflow are established to rapidly assess the Pb sources using the unique stable Pb isotopes fingerprints related to their geological sources. Deviation from the EPA’s method of one liter first draw tap water to sequential sampling (50-ml intervals) revealed more information about Pb and other element sources in the water.

Stable Pb isotope ratio fingerprinting revealed that the major sources of Pb in tap water are derived from the Cu pipe, valves, water tank and fixtures. A false sense of security is developed when the Pb service line is replaced with polyvinyl chloride (PVC) in older homes constructed before 1986.