The NJ Mass Spectrometry Discussion Group presents the May 2013 Meeting on Tuesday, May 7, 2013 at the Holiday Inn Somerset-Bridgewater, 195 Davidson Ave, Somerset NJ 08873. Sponsored by Agilent Technologies.
Attendance is free of charge, compliments of our sponsors!
Please register here.
I. Xinping Fang, Ph.D
VP, Head of Bioanalytical & Acting Head of Drug Metabolism / Biotransformation, XenoBiotic Laboratories, Inc, Plainsboro, NJ.
“Implementing an Ultrasensitive and Advanced New LC-MS/MS Platform, Xevo TQ-S, in a Regulated BA Lab”
II. Paul Rainville
Senior Manager of Scientific Operations, ESD, Pharmaceutical & Life Sciences Business Operations. Waters Corporation
“Advances in Front-End Technologies for Bioanalysis”
5:30 pm Social and registration
6:15 pm Complimentary dinner
7:00 pm Welcome and opening remarks
7:05 pm Xinping Fang
8:00 pm Paul Rainville
8:55 pm Closing remarks
Abstract for Talk II:
Modern bioanalytical chemists are charged with the task of developing high sensitivity assays for drug candidates with low circulating concentration, typically in the single pg/mL range. If this challenge was not difficult enough new economic, study design and ethical pressures demand that increasingly small volumes of samples are required and new sample formats such as dried blood spots. In this paper we will discuss the relative merits and pitfalls of two-dimensional liquid chromatography for bioanalytical applications when coupled to tandem quadrupole MS. Two-dimensional chromatography was evaluated for two applications i) to improve analyte cleanliness, thus increasing assay sensitivity and ii) to allow the direct injection of organic solvents onto a reversed phase system. As part of the study the mode of 2D operation was evaluated; forward flush, back flush and heart cut. Also we will discuss the use of a micro fabricated ceramic device packed with sub 2ìm for the analysis of plasma, bile and urine samples. We will show how the use of the micro scale separations device has been employed for the analysis of both small and large molecules.