Jared B. Shaw, Department of Chemistry, University of Nebraska-Lincoln

New approaches for top-down and native top-down protein characterization

Jared B. Shaw

Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE

Abstract: Bottom-up proteomics is a very sensitive and high throughput approach to proteome characterization; however, digestion of the proteome into peptides prior to analysis eliminates a vast amount of the biological context that can be obtained. Top-down and native top-down mass spectrometry enable direct characterization of the combinatorial modifications that yield proteoforms and the assembly of proteoforms into cellular machinery. Here, we will discuss recent developments in tandem mass spectrometry (MS/MS) instrumentation and methods in combination with other technologies, such as ion mobility spectrometry, that enable more efficient and accurate top-down characterization of sequence, posttranslational modifications (PTMs), and higher-order structure.

Bio: Jared Shaw is an assistant professor at the University of Nebraska-Lincoln in the Department of Chemistry. His research focuses on the development of novel mass spectrometry instrumentation and methods for the characterization of proteins and protein complexes by top-down (TD) and native top-down (nTD) mass spectrometry. Current interests include the application of these techniques to de novo antibody discovery and characterization of ribonucleoprotein complexes, such as the ribosome, and other large heterogeneous systems. Before moving to the University of Nebraska, Jared was a Post Doc and Senior Scientist at Pacific Northwest National Laboratory (PNNL, 2013-2020) and the Chief Scientific Officer at e-MSion, Inc. (2020-2023).