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Mar 23, 2021 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our March 2021 Meeting — Online!

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:.....Tuesday March 23, 2021

Time:.....7:00 - 8:15 PM

Via:......Webex

Cost:.....Free

Join:.....[ below ]

Sponsor:..Shimadzu

Shimadzu Scientific Instruments

Shimadzu Scientific Instruments cordially invites you to join us for an online talk on improving throughput in your laboratory.

Program

Speakers:  Stephen Kurzyniec

Pharma/Biopharma Business Development Manager, Shimadzu Scientific Instruments

“Mass Spec Approaches to Supporting Biologic Development”

Abstract:

Abstract: Monoclonal antibodies (mAbs) have become the most common modality for Biopharma therapeutic research programs. Years of research and development are required to identify the best candidate to move forward into clinical trials. Along the way, many analytical assays are used to screen, characterize and quantify these biomolecules. Traditionally, immunoassays such as ELISA (enzyme-linked Immunosorbent assay) have been the preferred analytical method, but more techniques have been stepping up to the challenge. Since the unique critical reagents required for ELISA can take months to generate, analytical techniques that take less method development time can have an “early-adoption” advantage that speeds up critical decision-making. Among them, LC-MS is the most appealing due to the accuracy, sensitivity, and specificity achieved by it.

In this presentation, we will discuss workflows to aid in the development, characterization and quantitation monoclonal antibodies. Shimadzu’s Cell Culture Media analysis provides bioprocess development scientists a 125-component snapshot in a single 17-minute LCMSMS method. Coupled with Protein Metrics Byos software, Shimadzu’s 9030 LCMS-QToF can provide an in-depth assessment of a mAb’s overall composition. Finally, Shimadzu’s nSMOL immunocapture kit for the quantitation of traditional mAbs, biosimilars, and hybrids by LC-MS/MS. Along with the nSMOL kit, the Ultra Fast scanning (30000 u/sec) and extreme sensitivity of the Shimadzu LCMS-8060 system allows for the detection of many CDR peptides from a single mAb or even for multiplexed samples in a single analytical run. The minimal method development time of nSMOL coupled with mass spectrometry can be a game-changer for mAb analysis in a high-throughput laboratory.

Pre-registration is not required.  Just join at the appointed time

NJACS MSDG invites you

to a Webex meeting.

“Mass Spec Approaches to Supporting Biologic Development”

Host: NJACS MSDG

Tuesday, March 23, 2021

7:00 pm | Eastern Time | 1 hr 15 mins

JOIN MEETING

Meeting number: 182 604 4539

Password: KSem85aCfg8

(57368522 from phones and video systems)

Join by phone +1-510-338-9438  (USA Toll)

Access code: 182 604 4539

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✎ 2021-03-23

(Past Events)

Sep 22, 2020 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our September 2020 Meeting — Online!

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:.....Tuesday September 22, 2020

Time:.....7:00 PM

Via:......Webex

Cost:.....Free

Join:.....[ below ]

Sponsor:..Novatia

Novatia LLC

Program

Speakers:  Robert Schuster and Kevin McCarl

Novatia, LLC Newtown, PA

“Automated deconvolution and analysis of intact biomolecule MS data: From Antibody drug conjugates to oligonucleotides”

Abstract:
Abstract: ProMass is the only fully automated mass spectrum deconvolution software package with versatile integration into ThermoFisher, Waters and SCIEX instrument operating platforms and a wide array of applications.  ProMass can be used to analyze the mass spectra of oligonucleotides, proteins, monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), small molecules and a variety of polymeric materials.  This webinar will focus on the application of ProMass to characterize antibody drug conjugates and oligonucleotides.  One study is the application of ProMass in conjunction with a Waters qTOF mass spectrometer to characterize lysine- and cysteine- antibody drug conjugate standards produced in-house, including automated DAR calculation.  In another study, ProMass HR was partnered with a Thermo Orbitrap to isotopically resolve oligonucleotide standards and a Thermo LTQ XL to develop a high throughput platform for rapid oligonucleotide quality control analysis.
Bio for: Robert Schuster, Ph.D.
Bio: Robert Schuster holds a B.S. in Biochemistry from Oakland University (2008), an M.S. in Chemistry from Tufts University (2011) and a Ph.D. in Medicinal Chemistry from Purdue University (2016). During his time at Purdue University, Rob worked with Laurie L. Parker and Jean-Christophe Rochet in the study of intracellular tyrosine kinase activation in Parkinson’s disease and Chronic Myeloid Leukemia, using exogenous peptide-based substrates and mass spectrometry (MS) quantitation. This work resulted in a patent on the development of degradation resistant peptide substrates to measure tyrosine kinase activation. As a Senior Research Fellow (Postdoctoral research associate) with Jing Zhang, at the University of Washington School of Medicine, he worked on protein biomarker identification, and the development of MS-based quantitative assays to analyze total protein and PTMs in blood and cerebrospinal fluid of patients diagnosed with Parkinson’s disease. Rob developed chemical derivatization methods to improve the sensitivity of assays measuring abundantly low peptides. Rob joined Novatia as a Senior Scientist in April 2018.
Bio for: Kevin McCarl, B.S.
Bio: Kevin received his B.S. in chemistry from the University of Pittsburgh (2016), where he did research under the direction of Dr. Stephen Weber. The bulk of Kevin’s research involved investigating the relationship between column temperature, mobile phase composition, and solute retention using HPLC and various stationary phases. Also during his time at Pitt, he designed and implemented a group research project which utilized GC-MS to determine the relative nicotine and carcinogen content in E-cigarette juices. Kevin joined Novatia in October 2016 as an Associate Research Scientist to provide and improve Novatia’s LC-MS platform services for biomolecules. He was was promoted to Research Scientist in early 2018 and has since expanded his analysis efforts. He also maintains the Novatia website and manages the company’s marketing efforts.

Pre-registration is not required.  Just join at the appointed time

NJACS MSDG invites you to a Webex meeting.

“Automated deconvolution and analysis of intact biomolecule MS data: From Antibody drug conjugates to oligonucleotides”

Host: NJACS MSDG Tuesday, September 22, 2020 7:00 pm | Eastern Time | 1 hr 15 mins

JOIN MEETING

Meeting number (access code): 142 133 8594

Meeting password: JXqpjdAD697 (59775323 from phones and video systems).....

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✎ 2020-09-22

(Past Events)

Jun 24, 2020 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our June 2020 Meeting — Online!

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:.....Wednesday June 24, 2020

Time:.....7:00 PM

Via:......Webex

Cost:.....Free

Register:.[ below ]

Sponsor:..Bruker

Program

Speaker:  Jarrod A. Marto, PhD

Principal Investigator, Dept. of Cancer Biology Dept. of Oncologic Pathology, Director, Blais Proteomics Center, Dana-Farber Cancer Institute Associate Professor, Dept. of Pathology, Brigham and Women’s Hospital and Harvard Medical School Boston, MA

“Leveraging the timsTOF Pro Platform to Interrogate the Functional Proteome”

Abstract:

Abstract: Despite the growing use of mass spectrometry in biomedical research, the wide dynamic range of protein expression and varying stoichiometry of post-translational modifications represent a significant hurdle to achieving ‘unbiased’ proteome characterization. These limitations also impede our ability to identify and characterize new protein drug targets. In this presentation I will discuss our recent efforts to leverage the unique analytical performance of the timsTOF Pro platform to interrogate multiple aspects of the functional proteome. Examples will include deep proteome coverage of highly enriched, primary human cells, analysis of post-translational modifications, characterization of selective small molecule inhibitors, as well as our initial efforts to develop parallel reaction monitoring (PRM) acquisition on the timsTOF Pro for biomarker validation.


Registration

NJACS MSDG invites you
to a Webex meeting that
requires registration.

“Leveraging the timsTOF Pro Platform to Interrogate
the Functional Proteome”

Host: NJACS MSDG
Wednesday, June 24, 2020
7:00 pm | Eastern Time | 1 hr 15 mins

Click to Register



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✎ 2020-06-24

(Past Events)

Mar 10, 2020 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our March 2020 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday March 10, 2020

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     908-707-1545

     [ http://somervilleelks.org ]

Sponsor: Agilent Technologies

Agilent

Please  register here.  Registration is free, compliments of our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Jim Arndt, PhD

Applications Scientist, MOBILion Systems, Inc.

“Structures for Lossless Ion Manipulations (SLIM) for the Masses”

Abstract for Jim Arndt:

Abstract: Structures for Lossless Ion Manipulation (SLIM) is a high-resolution ion mobility technique that enables ion packets to be transmitted around corners enabling several meters of separation in a small footprint without ion loss during separation1-3. In the last year, MOBILion Systems, Inc. has developed, and will commercialize, a thirteen-meter SLIM device coupled exclusively to Agilent Q-ToF systems. In our product overview seminar we will dive into our technical development and innovation efforts that are leading to the commercial product, with emphasis on proof-of-concept data in life science markets. Discussions will include lipidomics, clinical research testing, and biologic therapeutic characterization.

Speaker 2:  Qing Wang, PhD, MHS, MS

Founder and CEO, Complete Omics Inc.

“Direct Detection and Quantification of Neo-antigens”

Abstract for Qing Wang:

Abstract: Many immunotherapeutic approaches under development rely on T-cell recognition of cancer-derived peptides bound to human leukocyte antigen molecules on the cell surface. Direct experimental demonstration that such peptides are processed and bound is currently challenging. Here we described a method that meets this challenge. The method entailed an optimized immuno-precipitation protocol coupled with two-dimensional chromatography and mass spectrometry. The ability to detect and quantify minute amounts of pre-defined antigens should be useful for both basic research in tumor immunology as well as for the development of rationally-designed cancer vaccines.

✎ 2020-03-10

(Past Events)

Oct 9, 2019 – MSDG Monthly Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our October 2019 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Wednesday October 9, 2019

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908)707-1545

Sponsor: Bruker

Bruker Daltonics

 
Please  register here.  Registration is free, compliments of our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Prof. Peter Jackson, Ph.D.

Stanford University

“Cancer Mechanisms and Profiling from Analysis of AP/MS and Shotgun Proteomics”

Abstract for Peter Jackson:

One fifth of human cancers contain mutations activating the Ras GTPases, but no targeted therapies are yet available. Many effectors of the Ras pathway and potential targets remain underexplored and we have limited understanding of numerous paralogs of Ras effectors and other redundancies that contribute to Ras tumor growth. Here, we produce a multiomic map of the Ras function in lung adenocarcinoma. We use affinity purification mass spectrometry (AP/MS) to generate a protein-protein interaction (PPI) network. From this network, we constructed a sgRNA library covering 120 Ras pathway genes and screened for genetic interactions (GIs) by CRISPR dual knockout. We find new effectors of Ras-driven cellular function, and over 250 synthetic lethal GIs, presenting new candidate single and combination therapeutic targets. We identify important synergies between Ras and Rap signaling and between their downstream effector pathways. Our recent studies have used selective covalent inhibitors of a KRAS-G12C allele from the Shokat lab to address global signaling changes by shotgun proteomics, using the Bruker timsTOF. I will present the latest technical details for our data and also outline our informatics pipeline, which provides critical validation of our MS findings. These discoveries illustrate the power of multiomic interaction-mapping approach and may be applied to analyze a range of human diseases or other biological systems.

Speaker 2:  Tharan Srikumar, Ph.D.

Bruker Daltonics

“New Dimensions in Confidence for OMICS and HCPs with TIMS Technology”

Abstract for Tharan Srikumar:

Confidence in mass spectrometry measurements is key for advancing drug discovery projects. Bruker provides a comprehensive line of ESI and MALDI TOF instruments as well industry leading ultrahigh resolution MRMS systems and the evolutionary timsTOF Pro. The timsTOF Pro with PASEF relies on a unique dual Trapped Ion Mobility Spectrometry (TIMS) front end to provide nearly 100% duty cycle with no loss of ions. When combined with the Parallel Accumulation Serial Fragmentation (PASEF) method, the timsTOF Pro with PASEF enables sequencing speeds in excess of 100 Hz, and significant improvements in sensitivity. Powered by TIMS, the instrument provides separation for simple isomers and conformers and stands as an integrated part of 4D omics workflows because of the accurate and reproducible collisional cross-sections (CCS) values for all peptides. Recently, the TIMS technology has been applied to host cell protein (HCP) analysis of mAbs with record speed and depth. Newly announced diaPASEF enables more ways to accurately quantify proteins. The presentation will show how the technology can benefit HCP analysis and OMICS to step up the research to the next level.

✎ 2019-10-09

(Past Events)

Tue Sep 17, 2019 – MSDG Vendor Show & Symposium

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our September 2019 Annual Vendor Show and Symposium.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. 

Date:  Tuesday,  September 17, 2019

Venue: Somerville Elks Lodge 1068
       375 Union Avenue
       Bridgewater, NJ 08807
       908-707-1545
       www.somervilleelks.org

Please  register here.  Registration is free, compliments of our sponsors.

A limited number of free drink tickets will be provided to early attendees!

The Vendor Show features the following sponsors / vendors / collaborators on site (listed in the order of registration):
  JEOL         SciEx         908devices Protein Metrics   Agilent Peak Scientific IonBench   Shimadzu ThermoScientific Bruker IonSense Waters

Program

3:00 – 5:00 PM        Registration, Vendor Show, and Poster Session

5:00 – 5:45 PM        Prof Benjamin A. Garcia (University of Pennsylvania)

5:45 – 7:00 PM        Buffet Dinner (raffle prizes will be announced)

7:00 – 8:00 PM        Prof Vicki Wysocki (The Ohio State University)

Prof Benjamin A Garcia

 

Speaker 1: Prof Benjamin Garcia

University of Pennsylvania

“Quantitative Proteomics for Understanding the Epigenetic Cancer Code”

Abstract for Prof Garcia:

Histones are small proteins that package DNA into chromosomes, and a large number of studies have showed that several post-translational modification (PTM) sites on the histones are associated with both gene activation and silencing. Along with DNA and small non-coding RNA, histone PTMs make up epigenetic mechanisms that control gene expression patterns outside of DNA sequence mutations. Dysregulation of these chromatin networks underlie several human diseases such as cancer. Here I will give an update on technology advancements that have allowed for high-throughput quantitative analyses of histone PTMs and chromatin structure, and how we are applying these methods to understand epigenetic reprogramming found in malignant peripheral nerve sheath tumors (MPNSTs). MPNST is an aggressive sarcoma with recurrent loss of function alterations in polycomb-repressive complex 2 (PRC2), a histone-modifying complex involved in transcriptional silencing.


Prof Vicki Wysocki

Speaker 2: Prof Vicki Wysocki

The Ohio State University

“Native Mass Spectrometry and Surface-Induced Dissociation: Characterization of Protein and Nucleoprotein Complexes”


Abstract for Prof Wysocki:

Characterization of the overall topology and inter-subunit contacts of protein complexes, and their assembly/disassembly and unfolding pathways, is critical because protein complexes regulate key biological processes, including processes important in understanding and controlling disease. Tools to address structural biology problems continue to improve. Native mass spectrometry and associated technologies are becoming an increasingly important component of the structural biology toolbox. When the mass spectrometry approach is used early or mid-course in a structural characterization project, it can provide answers quickly using small sample amounts and samples that are not fully purified. Integration of sample preparation/purification with effective dissociation methods, ion mobility, and computational approaches provide a MS workflow that can be enabling in biochemical, synthetic biology, and systems biology approaches. Beyond what MS can provide as a stand-alone tool, MS can also guide and/or be integrated with other structural biology approaches such as NMR, X-ray crystallography, and cryoEM. MS can determine whether the complex of interest exists in a single or in multiple oligomeric states and can provide characterization of topology/intersubunit connectivity, and other structural features. Examples will be presented to illustrate the role MS and surface-induced dissociation can play in guiding a structural biology workflow and will include designed protein complexes and isolated or recombinant protein and nucleoprotein complexes.

✎ 2019-09-17

(Past Events)

May 7, 2019 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our May 2019 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday May 7, 2019

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908)707-1545

Sponsor: Waters

Waters
 
Please  register here.  Registration is free, compliments of our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Scott A. Robotham, Ph.D.

Research Investigator, Bioanalytical Sciences, Bristol-Myers Squibb

“Improving Biomarker Assay Workflows by Ensuring Reagent Quality using LC-MS Characterization”

Abstract for Scott A. Robotham:

The success of any assay is heavily dependent on the reagents used. For most assays this involves reagent antibodies, but in the case of biomarker assays recombinant proteins that act as a reference material for method development and QC are reagents that must be generated and characterized. Ensuring the quality of this reference protein is critical to the assay development, however oftentimes the only QC performed on these calibrator proteins is gel shift assay, which while useful only confirms if a protein is relatively close to the correct expected molecular weight. In order to provide a more accurate results, our group has utilized intact mass analysis to confirm amino acid sequences of calibrator proteins allowing for better quality control (QC) of these regents. In addition to this we have recently started to apply bottom-up proteomics techniques to our biomarker analysis in an effort to get additional information about these calibrator proteins. In this talk, two examples of how we are leveraging bottom-up to improve our reagent biomarkers workflows will be highlighted. The first example will explain how we are using proteomics to obtain sequence information for calibrator proteins that have amino acid sequences that are uncertain. For the second example our ongoing efforts to couple proteomics with immunocapture in order to characterize endogenous biomarkers will be discussed.

Speaker 2:  Weibin Chen, Ph.D.

Director, Scientific Operations, Waters Corporation

“Expanding Accessibility to High Performance LCMS Analytics in Biopharma: From Molecular Characterization to Attribute Monitoring”

Abstract for Weibin Chen:

Mass spectrometry (MS) has long been an enabling technology in the regulatory definition of a well‐ characterized biotherapeutic protein.  It is an invaluable tool to characterize molecular structures, assess process variabilities, and monitor the critical quality attributes of biotherapeutics – but until just recently has it been made accessible to biochemists.  Recent advances in LC, MS and informatics technologies are transforming biotherapeutic analyses, not only in terms of the quality of analytical data, but also in their accessibility for biochemists to generate, capture, and harness top‐quality LC/MS information to enable faster decision making.This talk will focus on how the evolving requirements of biotherapeutic analysis are addressed using modern LCMS analytical workflows built upon the recent introduced BioAccord system, and on how the leading separation, spectrometric, and informatics technologies are expanding the range of analytical challenges that MS workflows can routinely address while maintaining data integrity. We will provide several case studies on a panel of monoclonal antibodies (mAbs) for routine characterization and product quality attributes monitoring, and highlight how a workflow‐driven approach can produce time‐of‐flight mass spectrometry data that is not only highly comparable in quality to more complex advanced MS instruments, but that is more available to non‐traditional MS users in biopharmaceutical development and manufacturing organizations.

✎ 2019-05-07

(Past Events)

Apr 9, 2019 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our April 2019 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday April 9, 2019

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908)707-1545

Sponsor: Sciex

Sciex

Please  register here.  Registration is free, compliments of our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Dayanjan S Wijesinghe, Ph.D.

Assistant Professor, Department of Pharmacotherapy and Outcomes Sciences. Director, Laboratory of Pharmacometabolomics and Companion Diagnostics, Virginia Commonwealth University School of Pharmacy

“Lights Out Science: Harnessing advances in computer science to speed up biomedical research”

Abstract for Dayanjan Wijesinghe:

Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS) has been transforming the pace of biomedical research and discovery at an ever increasing pace over the past two decades. However, we are still challenged by the vastness of the data being generated and are unable to maximize all possible knowledge that can be gained from these experiments. Recent advances in computational methods have provided several tools that if used properly, can greatly increase the amount of clinically useful information that can be extracted from a single mass spectrometry assay. Employing such approaches will lead to a drastic reduction in the gaps in time between the acquisition of data, interpretation of results, formulation of new hypotheses and testing those new hypotheses. We hope to introduce the audience to some of those computational techniques available for use in LC-HRMS based biomedical research and to provide evidence of how these techniques are being used to gain new insights into previously unexplained disease pathologies.

Speaker 2:  Patty Sun

Global Market Manager, Pharma/CRO Quant/Met ID, SCIEX, Framingham MA, USA

“Confident Pharmaceutical Impurity Profiling using High-Res Mass Spec”

Abstract for Sandeep Kondaveeti:

In order to increase the safety and efficacy of drug products, impurity profiling is routinely performed to identify and quantify any residual impurities. These impurities may result from the instability of drug substances or reaction with added excipients or packaging materials or they may arise from starting and intermediate materials used in the manufacturing of active pharmaceutical ingredients (API). Since the amount of impurities will determine the safety and potency of the final product, a key challenge for drug development researchers is to identify, quantify, and control any impurities as early and efficiently as possible.

Because there can potentially be many impurities, some of which can be toxic and of low abundance, data processing can be onerous and time consuming if the software or the quality and type of data itself is not up to the task. Thus, an automated, high performance solution for detecting and identifying impurities is essential for relieving the burden and efficiently finding any stability or degradation issues.

The SCIEX X500R QTOF system and SCIEX OS Analytics were used to measure stability and detect impurities in amiodarone samples. Amiodarone is an anti-arrhythmic medication used to treat and prevent several types of irregular heartbeats. This integrated hardware and software solution compounds from MS and MS/MS data and reduces the need for manual review.

✎ 2019-04-09

(Past Events)

Mar 12, 2019 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our March 2019 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday March 12, 2019

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908)707-1545

Sponsor: Agilent

Agilent

Please  register here.  Registration is free, compliments of our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Adam Rosebrock, PhD

Assistant Professor, Department of Pathology, Stony Brook, NY

“Determining Antimicrobial Mode of Action by Targeted Ion Paired LC-MS”

Abstract for Adam Rosebrock:

Mass spectrometry metabolomics, the direct measurement of the biochemical content of cells, tissues and biofluids by GC- or LC-MS, is a maturing discipline with the potential to quantitate and identify a wide range of biological small molecules. Metabolomic measurements are orthogonal to genomics and proteomic read-outs, and provide a unique and complementary view of biological state.  In contrast to the static maps of reactions that adorn textbooks and wall-charts, metabolism is a dynamic, regulated process that changes across space, time, and cellular environment.

Although metabolomic analysis can be used to search for new reactions and metabolic intermediates, actionable biological information can be gained from measuring both levels flux of previously observed compounds.  I will describe how we perform “targeted” analysis using a combination of triple-quadrupole and high-resolution accurate-mass platforms to enable assays that are widely deployable in the research lab and beyond.  I will center my discussion around how my laboratory is using a combination or mass spectrometric measurement of small polar metabolites levels and flux to generate predictive metabolic profiles of response to environmental stimuli and to determine mechanism of action of antimicrobials.

Speaker 2:  Sandeep Kondaveeti

Applications Scientist, Agilent Technologies

“Intact Analysis of Biopharmaceuticals by Hydrophobic Interaction/Reversed Phase 2D-LC/MS System”

Abstract for Sandeep Kondaveeti:

Liquid Chromatography (LC) and Mass Spectrometry (MS) play essential role and are the dominant techniques used for characterizing mAb-based bio-therapeutics. Hydrophobic Interaction chromatography (HIC) is a popular LC technique widely used in downstream process purification but recently gained interest in analytical scale analysis of mAbs and ADCs.  HIC is a useful characterization tool because it can often separate mAb variants, for example certain isomers or oxidized species, which are often different to isolate from other orthogonal chromatography methods. It is also a popular tool in determining drug-to-antibody ratio (DAR) value, which is considered a Critical Quality Attribute (CQA) of an ADC product. Such investigations often benefit from peak identification using MS analysis, but high salt buffer conditions required for HIC separations make it incompatible to couple with online MS.  With the Agilent 2D-LC solution, the second dimension enables automated desalting by the addition of reversed-phase (RP) chromatography for a straightforward online LC/MS workflow. In this presentation, we demonstrate the analysis of mAbs and ADCs using multiple heart-cutting 2D-LC approach coupled with online Q-TOF MS system.

✎ 2019-03-12

(Past Events)

Dec 4, 2018 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our December 2018 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday December 4, 2018

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908)707-1545

Sponsor: Shimadzu

Shimadzu

Please  register here.  Registration is free, compliments to our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Jun Zhang

Principal Scientist, Bristol-Myers Squibb

“Evaluation of Microflow LC-MS/MS in a Quantitative Discovery Bioanalysis Setting”

Abstract for Jun Zhang:

Despite recent advances made in discovery bioanalysis to address the requirements of high throughput, high capacity and fast turnaround using LC-MS/MS, challenges in sensitivity remain for the analyses of some small molecules, peptides and other modalities. In theory microflow LC-MS/MS offers increased sensitivity compared to its regular flow counterpart due to more efficient MS ionization and sampling. In this study, the feasibility of using microflow LC-MS/MS for quantitative discovery bioanalysis was assessed. The evaluation demonstrated much improved sensitivity on microflow LC vs. regular flow LC for both small molecules and peptides, along with satisfactory analytical performance and robustness. The processes of method development, optimization and sample analysis on the microflow LC-MS/MS system were also shown to be straightforward. The better sensitivity enabled by microflow LC allowed for successful problem solving and method troubleshooting that was not feasible with regular flow LC in discovery bioanalysis.

Speaker 2:  Evelyn Wang; Ph.D.

Applications Scientist, Shimadzu Scientific Instruments

“Addressing the Challenges of Cell Culture Media Monitoring Through the Use of Triple Quad LCMS and TOF Analysis Techniques”

Abstract for Dr Wang:

Cell Culture monitoring can be challenging with all the contributing factors that can lead to cell line morbidity. As such the use of the proper tools while developing the monitoring technique is crucial to improve yields and cell line life. Once accomplished, the use of robust and reliable analysis tools to accurately map and develop intervention steps to increase the cell line growth and lifespan are crucial. This talk will discuss the merits of using the Shimadzu 9030 TOF platform for identification of components present as well as anomalies that may be observed from basal verses home grown cell line media and the use of the Shimadzu Triple Quad platform to routinely monitor cell line component consumption processes for accurate media monitoring. This process can then be transferred to manufacturing seamlessly through the use of C2MAP analysis using automated sample preparation followed by injection into the MS system for analysis.

✎ 2018-12-04

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