Mass Spectrometry Topical Group Meetings

375 Union Avenue, Bridgewater, NJ 08807

(908) 707-1545

Sponsor: NJ-MSDG

Please register here. It’s free, including dinner, but registration is required.

Masks or no masks are respected and welcomed.

Program

5:30 PM Social and Registration
6:15 PM Complimentary Dinner
6:55 PM Welcome and Opening Remarks
7:05 PM Speakers

Speaker 1: Srinivas Chakravartula, Ph.D, Mass Spectrometry Facility, Department of Chemistry and Chemical Biology. Rutgers, The State University of NJ

“Qualitative classification of tissue amyloidosis and subtyping by Laser Capture Microdissection (LMD) – LC-Mass spectrometry”

Abstract: Amyloidosis is a disease characterized by abnormal deposits of insoluble polymeric protein fibrils in tissue and organs. Condition of deposition of amyloid tissue is known as Amyloidosis. In my talk I will demonstrate an approach which simplifies, analysis of these deposits by utilizing high flow liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) of laser micro dissection (LMD) of Congo red +Ve amyloid deposits of formalin fixed paraffin embedded tissues (FFPE) from pathology preparations. The results presented demonstrate the validity of shotgun faster proteomics for precise and accurate identification and classification of amyloid subtypes that is practical for clinical application because of its shorter turnaround time bringing the state of the art pathological diagnosis of amyloidosis into the realm of routine surgical pathology practice to make the contribution of mass spectrometry even more powerful and meaningful for faster patient management decisions by clinicians and also for the effective treatment of amyloidosis. On the other hand, my LC-MS/MS assay is not just restricted for amyloid therapeutics but also opens the possibility of other tissues available to proteomics assays in studying other disease pathologies and for biomarker discoveries.

Speaker 2: Dr Gene Hall, PhD. Professor of Analytical Chemistry Department of Chemistry and Chemical Biology Rutgers, The State University of NJ

“Use of ICP-MS to Determine Sources of Pb in NJ Homeowner’s Tap Water: A Case for Sequential Sampling”

Abstract: We used an argon ion source to ionize the elements from Li to U in NJ homeowners, daycare centers, and elementary school tap water samples and plumbing materials. The ionized elements were sucked into a single quadrupole mass analyzer to determine concentrations and isotope ratios to determine Pb’s source(s) in the various samples. The combined methods are called inductively coupled plasma mass spectrometry (ICP-MS).

This seminar will focus on using ICP-MS to determine Pb’s source(s) in tap water samples collected from NJ homes, daycare centers, and elementary schools. A simple collect and inject workflow are established to rapidly assess the Pb sources using the unique stable Pb isotopes fingerprints related to their geological sources. Deviation from the EPA’s method of one liter first draw tap water to sequential sampling (50-ml intervals) revealed more information about Pb and other element sources in the water.

Stable Pb isotope ratio fingerprinting revealed that the major sources of Pb in tap water are derived from the Cu pipe, valves, water tank and fixtures. A false sense of security is developed when the Pb service line is replaced with polyvinyl chloride (PVC) in older homes constructed before 1986.

✎ 2023-06-27

(Past Events)

May 16, 2023 – MSDG Meeting

The NJ Mass Spectrometry Discussion Group is pleased to announce our May 16, 2023, Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date: Tuesday, May 16, 2023

375 Union Avenue, Bridgewater, NJ 08807

(908) 707-1545

Sponsor: Sciex

Please register here. It’s free, including dinner, but registration is required.

Masks or no masks are respected and welcomed.

Program

5:30 PM Social and Registration
6:15 PM Complimentary Dinner
6:55 PM Welcome and Opening Remarks
7:05 PM Speakers

Speaker 1: Eric Chan, PhD, Associate Director, Proteomics, HotSpot Therapeutics, Inc.

“Chemoproteomic workflow for target identification of small molecule allosteric inhibitors”

Abstract: HotSpot Therapeutics, Inc. is pioneering the discovery and development of a new class of allosteric drugs that target certain naturally occurring pockets on proteins called “natural hotspots”. These pockets are decisive in controlling a protein’s cellular function, and we believe they have significant potential for new drug discovery by enabling the systematic design of potent and selective small molecules with novel pharmacology.

We present our implementation of microflow LC, Q-TOF-MS and Zeno MS/MS for routine intact protein analysis, label-free PTM characterization, and isobaric tag-based proteomic profiling. To maximize throughput and to minimize downtime, all protein and proteomic workflows are carried out on the same trap-elute LC system and with a single ionization source. LC-MS complements our suite of biophysical, biochemical and modeling tools for the in vitro characterization of protein-ligand binding. Furthermore, when coupled with structure-based design of chemical probes, microflow LC and Zeno-MS/MS enabled the characterization of target engagement in live cells with performance comparable to nanoLC-MS3 and produced physiologically relevant information that expedited hit-to-lead progression in our drug discovery efforts.

Speaker 2: David Colquhoun, PhD, Market Development Manager

“Novel approaches to pharmacoproteomics analysis: higher throughput and deeper characterization”

Abstract: Proteomics analysis in drug discovery and development requires a considerably different approach compared to classical discovery approaches. This technique demands high throughput measurement of hundreds or thousands of samples, high inter-instrument and quantitative reproducibility and site-specific characterization of post-translational modifications. This presentation will illustrate approaches to deepen proteome coverage in shorter run times and increase quantitative robustness. We will discuss methods for identifying site specific modifications, novel PTMs and the technology that underpins these advancements.

✎ 2023-05-16

(Past Events)

April 25, 2023 – MSDG Meeting

The NJ Mass Spectrometry Discussion Group is pleased to announce our April 25, 2023 Meeting — Online!

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:.....Tuesday April 25, 2023

Time:.....7:00 PM | (UTC-05:00) Eastern Time (US & Canada)

Via:......Webex

Cost:.....Free

Join:.....[ below ]

Sponsor:..MOBILion Systems

Program

Speaker 1: Ron Orlando, PhD, Complex Carbohydrate Research Center; Department of Biochemistry & Molecular Biology and Chemistry; University of Georgia

“HILIC/MS – It’s not just for Glycans. Identification of Glycopeptides and Modified Peptides with the Combination of MS and HILIC Retention Modeling”

Abstract: Many of the chemical modifications that play a role in biological processes and protein aging often change the hydrophilicity of the modified amino acid’s side chain. These alterations can include the attachment of a single (or multiple) monosaccharides, oxidation of methionine, the deamidation of asparagine, and isomerization of aspartic acid, among others. Using conventional reverse-phase approaches, the peptides with and without these modifications often do not exhibit sufficient separation from one another, or when these can be chromatographically resolved the shift in retention is easily predictable. Changes in hydrophilicity directly alter a peptide’s retention on hydrophilic interaction liquid chromatography (HILIC) columns.

We have created a HILIC model that can predict the retention of peptides with various modifications. We have shown the ability to chromatographically resolve and quantitate products of deamidation, isomerization, oxidation, O-GlcNAcylation, and glycation. We have derived retention coefficients for these modified amino acids, and have incorporated them into a model that predicts peptide retention on HILIC columns.

The analysis of an extensive range of procainamide tagged N-linked glycans has led to a HILIC retention model for this class of biomolecule. The N-glycan model was combined with the peptide model, described above, to produce a prediction model for glycopeptides. The efficacy of the combined model was evaluated by comparing experimental data obtained via LC-MS analysis of human serum IgGs to those calculated with the model.

We feel that the unified chromatographic model (amino acids, modified amino acids, N-linked glycans) will facilitate the identification and quantification of peptides containing these modifications.

Speaker 2: Heidi Vitrac, Sr. Market Development Scientist, MOBILion Systems

“Unraveling the Released N-Glycan Isomerome Complexity by Implementing High-Resolution Ion Mobility (HRIM) in the HILIC-MS Workflow”

https://njacsmsdg.my.webex.com/njacsmsdg.my/j.php?MTID=me7c90d54cf0f446b00f0f0cb79bd5a3e

Abstract: The N-linked glycan moieties attached to therapeutic monoclonal antibodies (mAbs) can affect protein stability, bioactivity, and immunogenicity. Simple, biantennary N-linked glycan structures are readily assessed using simple fluorophore derivatization, online liquid chromatography, and fluorescence detection. However, traditional mAb-released N-linked glycan analysis results in a 15-60% ambiguity rate based on the inability to resolve isomers and reporting of compositions only. Furthermore, more complex protein biopharmaceuticals with glycosylation populations, such as Erythropoietin, highlight the need for tighter requirements for carbohydrate analysis, such as determining the antennal composition and elucidating of positional isomers. Here, we report that HRIM implementation in the workflow improves confidence in detecting and identifying N-linked glycan structures and isomers, while reducing data acquisition time.

InstantPC-labeled N-linked glycan biantennary standards were analyzed using a combination of flow injection analysis and hydrophilic interaction liquid chromatography (HILIC) separation. Data was acquired on an HRIM MOBIE™ instrument (MOBILion Systems) coupled to a 6545XT QTOF (Agilent Technologies). Accurate mass, isotope spacing, ion mobility arrival time distribution, and Collision Cross Section (CCS) determination were used to identify each N-linked glycan. Each alpha- and beta-linked N-linked glycan isomer structure was verified by analyzing individual standards. Data processing, analysis, relative quantification, and visualization were achieved using HRIM Data Processor, PNNL Preprocessor, and Protein Metrics Byos® Software.

First, flow injection analysis of 28 InstantPC-labeled N-linked glycan compositions (of which, 12 have isomeric linkages) was conducted to determine the HRIM-MS profiles of each N-glycan. For each labeled N-linked glycan, accurate mass, isotope spacing, ion mobility arrival time distribution, and Collision Cross Section (CCS) values were determined with a minimum of triplicate measurements. N-linked glycan detection, identity validation, and relative quantitation were conducted, generating an N-linked glycan feature library used in later HILIC-HRIM-MS analytical workflows. We demonstrate that HRIM-MS allows for the separation, confident identification, and quantification of all 28 N-linked glycan species. Further, all 12 N-linked glycan isomers could be resolved into multiple ion mobility peaks in the gas phase, without any LC separation. Rapid N-linked glycan profiling was further demonstrated by analyzing a human IgG-released InstantPC-labeled N-linked glycan library. Altogether, our flow injection results demonstrate that HRIM-MS offers excellent potential for rapidly and confidently revealing complex glycoform profiles.

Second, we implemented HRIM in the traditional HILIC-MS analytical workflow for released N-linked glycans, intending to test the complementarity of HILIC and HRIM separation. Varying HILIC run times (60-, and 15-minute gradients) were tested to assess the ability of HRIM to resolve N-linked glycans in the gas phase while HILIC separation times were gradually reduced. All three HILIC gradients resulted in comparable relative N-linked glycan species coverage and isomer separation, with consistent and reproducible relative quantitation based on extracted ion mobiligrams. Our results demonstrate that HRIM-MS provides high-quality and rapid N-linked glycan feature identification (including isomeric separation) and relative quantitation. Leveraging accurate and reproducible CCS determination, adding HRIM to a traditional LC-MS method provides a solution for confidently fingerprinting glycosylation profiles in biotherapeutics, enabling a greater depth in N-linked glycan feature detection or increased analytical throughput.

Three bullet points about the talk:

Traditional mAb-released N-glycan analysis results in a 15-60% ambiguity rate based on the inability to resolve isomers and reporting of compositions only.
HRIM-MS allows for the separation, confident identification, and quantification of released N-glycan species, including isomer resolution, without any LC separation.
Leveraging accurate and reproducible CCS determination, HRIM addition to a HILIC-MS workflow provides confident fingerprinting of glycosylation profiles in biotherapeutics while enabling increased analytical throughput.

Pre-registration is not required. Just [ join ] at the appointed time

NJACS MSDG invites you

to a Webex meeting.

NJ-ACS Mass Spectrometry Discussion Group Virtual Meeting

Tuesday, April 25, 2023

7:00 pm | Eastern Time | 1 hr 15 mins

More ways to join:

Join with the full meeting link:

Or use this shortcut link:
https://njacs.org/msdg-webex

Join by meeting number:

Meeting number (access code): 2558 596 0838

Meeting Password: yxPDtiEC822

(also 99738432 from phones and video systems)

Join by phone (voice only):

+1-510-338-9438 (USA Toll)

Access code: 2556 896 6928

Or by a single tap on a mobile phone (voice only):

+1-510-338-9438,,25568966928 (USA Toll)

Join by video system, application or Skype for business

Dial 1825116738@webex.com

You can also dial 173.243.2.68 and enter the meeting number.

Need help? Go to https://help.webex.com

✎ 2023-04-25

(Past Events)

April 11, 2023 – MSDG Meeting

The NJ Mass Spectrometry Discussion Group is pleased to announce our April 11 2023 Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date: Tuesday, April 11, 2023

375 Union Avenue, Bridgewater, NJ 08807

(908) 707-1545

Please register here. It’s free, including dinner, but registration is required.

Masks or no masks are respected and welcomed.

Program

5:30 PM Social time (cash bar / appetizers)
6:15 PM Complimentary Dinner
6:55 PM Welcome and Opening Remarks
7:05 PM Invited Speakers

Speaker 1: Wendy Zhong, PhD, Sr. Principal Scientist, Analytical Research & Development, Merck Research Laboratories, Rahway, NJ, USA

“Label Free Molecular Imaging and Electron-based Dissociation via High Resolution Mass Spectrometry—Applications in Pharmaceutical Industry”

Abstract: With the increased complexity of new and diverse modalities in the pharmaceutical industry, there is a high demand to develop more sensitive and versatile analytical tools. HRMS has become an important analytical technique due to its broad applications in small molecule structure identification, molecular imaging, large biomolecules such as protein and antibody-drug conjugate characterization. In this presentation, I will discuss several unique/innovative approaches we developed and applied to solve challenging problems in drug discovery and development.

• Label free molecular imaging applications in tissues and tablets

Fatty liver disease phenotype is characterized by both an increase in the concentration and synthesis rate of neutral lipids (NL) across the liver. The spatial distribution of multiple lipids classes is poorly characterized in fatty liver disease. A novel method was development to enhance ionization of neutral lipid via Sodium-Doped Gold-Assisted Laser Desorption Ionization. This method can allow investigators to obtain spatial resolution of lipogenic flux by coupling imaging mass spectrometry and isotope tracers. Long-acting injectable (LAI) implants can deliver a drug over several weeks to years, reducing the dosing frequency and improving patient adherence. An innovative approach of using MALDI-MSI to characterize the drug release process from LAI implants was developed. This method provides definitive molecular-level information about the chemical composition as well as the distribution of APIs simultaneously.

• Development of Electron-based dissociation (ExD) to differentiate isomeric amino acids

Isomeric amino acids such as aspartic isoaspartic acid leucine/isoleucine, and valine/norvaline are widely present in peptides and proteins. For example: Leu and Ile count for 16% of all amino acids in proteins. Nva differs from Leu by only one methyl group, and mis-incorporation of Nva is common during the production of recombinant proteins. Conventional CID generates the same m/z value for these isomers and consequently is unable to differentiate isobaric species. Therefore, it poses a challenge to establishing correct peptide and proteins sequences. Electron-activated dissociation (ExD) was developed to differentiate these isomeric amino acid residues in therapeutic peptide and protein.

Speaker 2: Ethan Yang, PhD, Applications Lead, Imaging/MRMS, Bruker Scientific LLC, Billerica, MA USA

“Recent Advances in High Spatial Resolution MALDI Imaging for Metabolomics and Proteomics with the Bruker timsTOF fleX MALDI-2 Platform”

Abstract: Recent innovations in instrumentation in the Bruker MALDI imaging platform—the timsTOF fleX MALDI-2 with microGRID—are now enabling deeper biological investigations in small metabolites and large proteins. Specific examples of metabolic pathways and cancer biology with results as 5 µm spatial resolution are shown.

✎ 2023-04-11

(Past Events)

Nov 1, 2022 – MSDG Meeting

Under Construction

The NJ Mass Spectrometry Discussion Group is pleased to announce our November 2022 Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date: Tuesday, November 1, 2022

375 Union Avenue, Bridgewater, NJ 08807

(908) 707-1545

Please register here. Free but registration required. Includes dinner.

Masks or no masks are respected and welcomed.

Program

4:30 PM Social time (cash bar / appetizers)
6:00 PM Complimentary Dinner
7:00 PM Invited Speaker

The NJMSDG steering committee is delighted to welcome ASMS’s Archivist / Historian, P. Jane Gale, PhD to present

“Decade by Decade: An Historical Review of Mass Spectrometry and ASMS in the Second Half of the 20th Century“

P. Jane Gale, Ph.D. to present on Tuesday, November 1st 2022

Abstract: Following fifteen years of meeting as part of the American Society for Testing and Materials (ASTM, now ASTM International) a small group of influential mass spectrometrists broke away from the umbrella group to found the American Society for Mass Spectrometry. Whereas in the early days talks at annual get-togethers were dominated by topics related to the oil industry, the newly formed ASMS had a broader focus and included lively discussions of everything from mechanistic studies of molecules in the gas phase to surface science studies of materials important to the electronics industry. The growing Society became the premier US venue in which to present both fundamental research on the technique and applied research into its use in ever-widening fields of study. This talk will follow the Society’s growth and development over the last 70+ years, incorporating into that history the vast array of scientific developments that enabled new areas of application.

We will have a contest (with prizes) for the oldest working mass spectrometer! We’ll include all pictures in a slide show. Please send pictures and provenances/details to ccabral@njacs.org. You must be present to win but, but, but, you can submit any ‘known to you’ MS with proper details. So reach back to those university instruments where you eked out that data for your PhD, etc, . . . love to have your pics and stories . . . but it must still be working – no old unit sitting in storage waiting for someone to figure out how to dispose of it 😊. This will be like ‘baby picture’ time in reverse.

✎ 2022-11-01

(Past Events)

April 12, 2022 – MSDG Meeting

The NJ Mass Spectrometry Discussion Group is pleased to announce our April 12, 2022 Meeting — Online!

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:.....Tuesday April 12, 2022

Time:.....7:00 PM | (UTC-05:00) Eastern Time (US & Canada)

Via:......Webex

Cost:.....Free

Join:.....[ below ]

Sponsor:..Bruker

Program

Speaker 1: Hanno Steen, PhD, Director of Proteomics and Associate Professor, Department of Pathology, Boston Children’s Hospital and Harvard Medical School, Boston, MA, USA

“Scaling up while scaling down – Sample-sparing High Throughput Proteomics”

“Ultra-sensitive 4D Proteomics on the timsTOF SCP mass spectrometer”

Abstract: Since its inception, proteomics was poised to revolutionize clinical diagnostics by providing novel biomarkers. Currently, however, proteomics has not met its own expectations particularly because large population sizes are required for meaningful biomarkers that can account for genetic, environmental and lifestyle variance. A main bottleneck has been the suboptimal throughput. Enabling throughput will allow the robust processing and analysis of statistically meaningful samples numbers, i.e., hundreds to thousands of samples within a reasonable timeframe. Our recent, involvement in several NIAID immunophenotyping studies focused on understanding COVID-19 impact on the immune system, the efficacy of vaccination, and ontogeny in early life with thousands of plasma samples, highlighted the importance of increasing throughput in collecting high quality data while simultaneously decreasing the amount of required starting material. The Steen Lab has developed a high throughput proteomics platform that allows for the robust processing and analysis of such large numbers of plasma samples. These efforts leveraged the methodology and experience of the Steen Lab to process and analyze hundreds of urine samples with the aim of identifying non-invasive biomarker candidates. The presentation will describe method development from the Steen Lab towards i) high throughput proteomics, ii) improved depth of the plasma proteome, and iii) meta-analyses converting many small studies and datasets into a large dataset with statistical relevance. Our platform which incorporates these approaches will enable the analysis of small volumes of large sample numbers which will bring allow us to understand deviations from normal despite natural interpersonal variability, i.e., truly personalized medicine.

Speaker 2: Matt Willets, US Proteomics Applications Manager, Bruker

https://njacsmsdg.my.webex.com/njacsmsdg.my/j.php?MTID=m1e203489cf1f1889aa56b4f2851a4b60

Abstract: The timsTOF SCP is the first commercially introduced mass spectrometer for single cell proteomics. The modified front end (orthogonal ion guide) and the brighter ion beam increase the ion transfer up to five times while maintaining instrument high robustness. In this presentation, we will show how the ultra-high sensitivity of the timsTOF Pro can benefit workflows from immunopeptidomics to single cell analysis

Pre-registration is not required. Just join at the appointed time

NJACS MSDG invites you

to a Webex meeting.

NJ-ACS Mass Spectrometry Discussion Group Virtual Meeting

Tuesday, April 12, 2022

7:00 pm | Eastern Time | 1 hr 15 mins

More ways to join:

Join from the meeting link:

Join by meeting number:

Meeting number (access code): 2556 896 6928

Meeting Password: 6543212

(also 654321 from phones and video systems)

Join by phone (voice only):

+1-510-338-9438 (USA Toll)

Access code: 2556 896 6928

Or by a single tap on a mobile phone (voice only):

+1-510-338-9438,,25568966928 (USA Toll)

Join by video system, application or Skype for business

Dial 1825116738@webex.com

You can also dial 173.243.2.68 and enter the meeting number.

Need help? Go to https://help.webex.com

✎ 2022-04-12

(Past Events)

December 8, 2021 – MSDG Meeting

The NJ Mass Spectrometry Discussion Group is pleased to announce our December 8, 2021 Meeting — Online!

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:.....Wednesday December 8, 2021

Time:.....7:00 PM | (UTC-05:00) Eastern Time (US & Canada)

Via:......Webex

Cost:.....Free

Join:.....[ below ]

Sponsor:..Waters Corporation

Program

Speaker 1: Dr. Ying Zhang, Pfizer

Streamlining and Accelerating Peptide Multi-Attribute Method (MAM) Analysis with a BioAccord MAM Workflow to Enhance Product Knowledge”

“Rapid Analysis of Synthetic mRNA Cap Structure Using Ion-Pairing RPLC with MaxPeak™ Premier Columns and the BioAccord LC-MS System”

Abstract: Development of biopharmaceuticals requires a thorough understanding of product quality attributes (PQAs) and impurities to ensure that the product meets desired safety and efficacy profiles. Multi-attribute method (MAM), based on LC-MS peptide mapping technology, enables direct site-specific monitoring of challenging PQAs and allows enhanced product and process understanding. In this presentation, we will demonstrate how we utilize a BioAccord MAM workflow to increase our product knowledge through both PQA monitoring and new peak detection.

Speaker 2: Dr. Jennifer Nguyen, Waters Corporation

https://njacsmsdg.my.webex.com/njacsmsdg.my/j.php?MTID=m3734dc989d819feed79b7f62cd39a2a9

Abstract: The 5’ cap of mRNA is vital to characterize in vaccine development. Ion-pairing reversed phase liquid-chromatography mass spectrometry (LC-MS) can be used to analyze pre-defined 5’ fragments of synthetic mRNA. However, accurate quantitation can be difficult to achieve due to non-specific binding with the system. Moreover, the choice of ion-pairing system can heavily influence the quality and sensitivity of the mass spectra. We developed a rapid and sensitive LC-MS method applicable to synthetic mRNA capping using MaxPeak™ Premier Columns in combination with the compliance-ready BioAccord LC-MS system. Analysis of 5’ capping of IVT mRNA preparations could be achieved in less than 5-minutes. Linearity was demonstrated to detecting product-related impurities down to less than 0.1% of the target 5’ capped fragment.

Pre-registration is not required. Just join at the appointed time

NJACS MSDG invites you

to a Webex meeting.

NJ-ACS Mass Spectrometry Discussion Group Virtual Meeting

Wednesday, December 8, 2021

7:00 pm | Eastern Time | 1 hr 15 mins

More ways to join:

Join from the meeting link:

Join by meeting number:

Meeting number (access code): 2559 427 5257

Meeting Password: axP5cDbSs22

(29752327 from phones and video systems)

Join by phone (voice only):

+1-510-338-9438 (USA Toll)

Access code: 2559 427 5257

Or by a single tap on a mobile phone (voice only):

+1-510-338-9438,,25594275257 (USA Toll)

Join by video system, application or Skype for business

Dial 1825116738@webex.com

You can also dial 173.243.2.68 and enter the meeting number.

Need help? Go to https://help.webex.com

✎ 2021-12-08

(Past Events)

August 11, 2021 – MSDG Meeting

The NJ Mass Spectrometry Discussion Group is pleased to announce our August 11, 2021 Meeting — Online!

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:.....Wednesday August 11, 2021

Time:.....7:00 - 8:15 PM

Via:......Webex

Cost:.....Free

Join:.....[ below ]

Sponsor:..SCIEX

Program

Speaker 1: Dr. Andrew Wagner

PhD, Principal Scientist Small Molecule Drug Discovery at Bristol Myers Squibb

“Breaking Throughput Barriers of Mass Spectrometry (MS)-Based Analysis Using Acoustic Ejection Sample Delivery: Impacts in Early Drug Discovery and Beyond”

“New novel developments in high resolution mass spectrometry to address lower levels of detection and improved characterization of analytes”

Abstract: Label-free, MS-based analysis is routinely used throughout drug discovery to help drive important decision-making and enable lead discovery, hit triaging and lead optimization. MS-based methodologies have traditionally relied upon liquid chromatography (LC) or on-line solid phase extraction (SPE) as front-end sample clean-up mechanisms. These approaches have slow cycle times (> 10 seconds/sample) that often aren’t amenable to high throughput screening assays conducted in 384- or 1536-well plates. Acoustic ejection mass spectrometry is a recently developed high-throughput MS approach that combines acoustic droplet ejection (ADE) with an open-port interface (OPI) to enable direct sample introduction from plates into the mass spectrometer for analysis. Using the acoustic ejection mass spectrometer system, we have demonstrated analytical throughput speeds as fast as 1 second-per-sample for multiple screening assays. This remarkable improvement in MS cycle time, approaching that of optical plate readers, has resulted in increased capacity and faster turn-around-time for reading many critical early drug discovery studies such as liability assessment and activity-based screens, and has the potential to impact many other areas within drug discovery.

Speaker 2: Dr. Jose Castro-Perez

Senior Director Accurate Mass Product Management at SCIEX

https://njacsmsdg.my.webex.com/njacsmsdg.my/j.php?MTID=m4d5315d73fb5ba10bf95e9ab1be241b6

Abstract: High resolution mass spectrometry has continued to grow as new application areas are constantly emerging. Here, we will present and discuss new developments which addresses both sensitivity and structural characterization needs for a variety of application areas for both small and large molecules in Life Sciences Multi-Omics and also in Drug Discovery..

Pre-registration is not required. Just join at the appointed time

NJACS MSDG invites you

to a Webex meeting.

NJ-ACS Mass Spectrometry Discussion Group Virtual Meeting

Wednesday, August 11, 2021

7:00 pm | Eastern Time | 1 hr 15 mins

More ways to join:

Join from the meeting link:

Join by meeting number:

Meeting number (access code): 182 511 6738

Meeting Password: WRn5e29HjnP

(97653294 from phones and video systems)

Join by phone (voice only):

+1-510-338-9438 (USA Toll)

Access code: 182 511 6738

Or by a single tap on a mobile phone (voice only):

+1-510-338-9438,,1825116738 (USA Toll)

Join by video system, application or Skype for business

Dial 1825116738@webex.com

You can also dial 173.243.2.68 and enter the meeting number.

Need help? Go to https://help.webex.com

✎ 2021-08-11

(Past Events)

July 21, 2021 – MSDG Meeting

The NJ Mass Spectrometry Discussion Group is pleased to announce our July 21, 2021 Meeting — Online!

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:.....Wednesday July 21, 2021

Time:.....6:30 - 7:45 PM

Via:......Webex

Cost:.....Free

Join:.....[ below ]

Sponsor:..Agilent

Program

Speaker 1: Dr. John Schiel

PhD, NIST Biomolecular Measurement Division

“Multi-Attribute Mass Spectrometry as a Multi-Modality Technology”

“Characterization and quantification of synthetic oligonucleotides using HRAM and QQQ”

Abstract: Characterization of mAbs has evolved during the past 30 years to include a toolbox of measurements to elucidate identity, quality, and stability. Molecular complexity is magnified with gene therapies, as is the need to pursue adaptation of high-resolution analytical measurement approaches to further the understanding and development of these novel modalities. This talk will describe the principles of the multi-attribute mass spectrometry method (MAM) as applied to mAbs and its evolution toward identification and control of viral vector quality attributes.

About Dr. Schiel:

Bio: Dr. Schiel received his BS (2004) and Ph.D. (2009) in chemistry from the University of Nebraska-Lincoln, and is currently a research chemist in the NIST Biomolecular Measurement Division. Dr. Schiel coordinates the development of Reference Materials that support the biomanufacturing industry, including the recombinant IgG1κ NIST monoclonal antibody Reference Material 8671 (NISTmAB). He also leads an analytical research team developing innovative approaches toward production/characterization of next generation biotherapeutics (e.g. viral vectors, cell therapy products, and vaccines) and de-risking of innovative technologies for lifecycle appropriate implementation and regulatory assimilation. He is an author of over 30 publications, an editor of the ACS book series “State-of-the-Art and Emerging Technologies for Therapeutic Monoclonal Antibody Characterization”, and recipient of numerous Awards including the Arthur S. Flemming Award, Department of Commerce Gold Medal, ACS Division of Analytical Chemistry Fellowship, Bioanalysis Young Investigator Award, and UNL Early Achiever Award.

Speaker 2: Peter Rye

Application Scientist, Agilent

https://njacsmsdg.my.webex.com/njacsmsdg.my/j.php?MTID=mfb241ec2bcd360aa25aaa41360c41b12

Abstract: Mass spectrometry plays a vital role in the measurement and characterization of synthetic DNA and RNA oligonucleotides (oligos), which are used widely in molecular biology and in the development of therapeutics. This webinar will highlight (1) the quantitative analysis of oligos, both by QQQ and HRAM technologies, to achieve the best combination of sensitivity and speed, (2) impurities determinations using HRAM, multiple analysis techniques, and purpose-built software, and (3) high-throughput methods that enable the acquisition, analysis, and purity review of over 5,000 samples a day. Join us to push the speed, sensitivity, and dynamic range of oligo measurements to new heights.

Pre-registration is not required. Just join at the appointed time

NJACS MSDG invites you

to a Webex meeting.

NJ-ACS Mass Spectrometry Discussion Group Virtual Meeting

Wednesday, July 21, 2021

6:30 pm | Eastern Time | 1 hr 15 mins

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Join from the meeting link:

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Meeting number (access code): 182 221 1293

Meeting Password: nFAMNdAX834

(63266329 from phones and video systems)

Join by phone (voice only):

+1-510-338-9438 (USA Toll)

Access code: 182 221 1293

Or by a single tap on a mobile phone (voice only):

+1-510-338-9438,,1822211293#63266329# (USA Toll)