North Jersey Section
American Chemical Society

Mass Spectrometry Topical Group Meetings

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(Recent Event)

May 7, 2019 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our May 2019 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday May 7, 2019

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908)707-1545

Sponsor: Waters

Waters
 
Please  register here.  Registration is free, compliments of our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Scott A. Robotham, Ph.D.

Research Investigator, Bioanalytical Sciences, Bristol-Myers Squibb

“Improving Biomarker Assay Workflows by Ensuring Reagent Quality using LC-MS Characterization”

Abstract for Scott A. Robotham:

The success of any assay is heavily dependent on the reagents used. For most assays this involves reagent antibodies, but in the case of biomarker assays recombinant proteins that act as a reference material for method development and QC are reagents that must be generated and characterized. Ensuring the quality of this reference protein is critical to the assay development, however oftentimes the only QC performed on these calibrator proteins is gel shift assay, which while useful only confirms if a protein is relatively close to the correct expected molecular weight. In order to provide a more accurate results, our group has utilized intact mass analysis to confirm amino acid sequences of calibrator proteins allowing for better quality control (QC) of these regents. In addition to this we have recently started to apply bottom-up proteomics techniques to our biomarker analysis in an effort to get additional information about these calibrator proteins. In this talk, two examples of how we are leveraging bottom-up to improve our reagent biomarkers workflows will be highlighted. The first example will explain how we are using proteomics to obtain sequence information for calibrator proteins that have amino acid sequences that are uncertain. For the second example our ongoing efforts to couple proteomics with immunocapture in order to characterize endogenous biomarkers will be discussed.

Speaker 2:  Weibin Chen, Ph.D.

Director, Scientific Operations, Waters Corporation

“Expanding Accessibility to High Performance LCMS Analytics in Biopharma: From Molecular Characterization to Attribute Monitoring”

Abstract for Weibin Chen:

Mass spectrometry (MS) has long been an enabling technology in the regulatory definition of a well‐ characterized biotherapeutic protein.  It is an invaluable tool to characterize molecular structures, assess process variabilities, and monitor the critical quality attributes of biotherapeutics – but until just recently has it been made accessible to biochemists.  Recent advances in LC, MS and informatics technologies are transforming biotherapeutic analyses, not only in terms of the quality of analytical data, but also in their accessibility for biochemists to generate, capture, and harness top‐quality LC/MS information to enable faster decision making.This talk will focus on how the evolving requirements of biotherapeutic analysis are addressed using modern LCMS analytical workflows built upon the recent introduced BioAccord system, and on how the leading separation, spectrometric, and informatics technologies are expanding the range of analytical challenges that MS workflows can routinely address while maintaining data integrity. We will provide several case studies on a panel of monoclonal antibodies (mAbs) for routine characterization and product quality attributes monitoring, and highlight how a workflow‐driven approach can produce time‐of‐flight mass spectrometry data that is not only highly comparable in quality to more complex advanced MS instruments, but that is more available to non‐traditional MS users in biopharmaceutical development and manufacturing organizations.

2019-05-07

(Recent Event)

Apr 9, 2019 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our April 2019 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday April 9, 2019

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908)707-1545

Sponsor: Sciex

Sciex

Please  register here.  Registration is free, compliments of our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Dayanjan S Wijesinghe, Ph.D.

Assistant Professor, Department of Pharmacotherapy and Outcomes Sciences. Director, Laboratory of Pharmacometabolomics and Companion Diagnostics, Virginia Commonwealth University School of Pharmacy

“Lights Out Science: Harnessing advances in computer science to speed up biomedical research”

Abstract for Dayanjan Wijesinghe:

Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS) has been transforming the pace of biomedical research and discovery at an ever increasing pace over the past two decades. However, we are still challenged by the vastness of the data being generated and are unable to maximize all possible knowledge that can be gained from these experiments. Recent advances in computational methods have provided several tools that if used properly, can greatly increase the amount of clinically useful information that can be extracted from a single mass spectrometry assay. Employing such approaches will lead to a drastic reduction in the gaps in time between the acquisition of data, interpretation of results, formulation of new hypotheses and testing those new hypotheses. We hope to introduce the audience to some of those computational techniques available for use in LC-HRMS based biomedical research and to provide evidence of how these techniques are being used to gain new insights into previously unexplained disease pathologies.

Speaker 2:  Patty Sun

Global Market Manager, Pharma/CRO Quant/Met ID, SCIEX, Framingham MA, USA

“Confident Pharmaceutical Impurity Profiling using High-Res Mass Spec”

Abstract for Sandeep Kondaveeti:

In order to increase the safety and efficacy of drug products, impurity profiling is routinely performed to identify and quantify any residual impurities. These impurities may result from the instability of drug substances or reaction with added excipients or packaging materials or they may arise from starting and intermediate materials used in the manufacturing of active pharmaceutical ingredients (API). Since the amount of impurities will determine the safety and potency of the final product, a key challenge for drug development researchers is to identify, quantify, and control any impurities as early and efficiently as possible.

Because there can potentially be many impurities, some of which can be toxic and of low abundance, data processing can be onerous and time consuming if the software or the quality and type of data itself is not up to the task. Thus, an automated, high performance solution for detecting and identifying impurities is essential for relieving the burden and efficiently finding any stability or degradation issues.

The SCIEX X500R QTOF system and SCIEX OS Analytics were used to measure stability and detect impurities in amiodarone samples. Amiodarone is an anti-arrhythmic medication used to treat and prevent several types of irregular heartbeats. This integrated hardware and software solution compounds from MS and MS/MS data and reduces the need for manual review.

2019-04-09

(Recent Event)

Mar 12, 2019 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our March 2019 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday March 12, 2019

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908)707-1545

Sponsor: Agilent

Agilent

Please  register here.  Registration is free, compliments of our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Adam Rosebrock, PhD

Assistant Professor, Department of Pathology, Stony Brook, NY

“Determining Antimicrobial Mode of Action by Targeted Ion Paired LC-MS”

Abstract for Adam Rosebrock:

Mass spectrometry metabolomics, the direct measurement of the biochemical content of cells, tissues and biofluids by GC- or LC-MS, is a maturing discipline with the potential to quantitate and identify a wide range of biological small molecules. Metabolomic measurements are orthogonal to genomics and proteomic read-outs, and provide a unique and complementary view of biological state.  In contrast to the static maps of reactions that adorn textbooks and wall-charts, metabolism is a dynamic, regulated process that changes across space, time, and cellular environment.

Although metabolomic analysis can be used to search for new reactions and metabolic intermediates, actionable biological information can be gained from measuring both levels flux of previously observed compounds.  I will describe how we perform “targeted” analysis using a combination of triple-quadrupole and high-resolution accurate-mass platforms to enable assays that are widely deployable in the research lab and beyond.  I will center my discussion around how my laboratory is using a combination or mass spectrometric measurement of small polar metabolites levels and flux to generate predictive metabolic profiles of response to environmental stimuli and to determine mechanism of action of antimicrobials.

Speaker 2:  Sandeep Kondaveeti

Applications Scientist, Agilent Technologies

“Intact Analysis of Biopharmaceuticals by Hydrophobic Interaction/Reversed Phase 2D-LC/MS System”

Abstract for Sandeep Kondaveeti:

Liquid Chromatography (LC) and Mass Spectrometry (MS) play essential role and are the dominant techniques used for characterizing mAb-based bio-therapeutics. Hydrophobic Interaction chromatography (HIC) is a popular LC technique widely used in downstream process purification but recently gained interest in analytical scale analysis of mAbs and ADCs.  HIC is a useful characterization tool because it can often separate mAb variants, for example certain isomers or oxidized species, which are often different to isolate from other orthogonal chromatography methods. It is also a popular tool in determining drug-to-antibody ratio (DAR) value, which is considered a Critical Quality Attribute (CQA) of an ADC product. Such investigations often benefit from peak identification using MS analysis, but high salt buffer conditions required for HIC separations make it incompatible to couple with online MS.  With the Agilent 2D-LC solution, the second dimension enables automated desalting by the addition of reversed-phase (RP) chromatography for a straightforward online LC/MS workflow. In this presentation, we demonstrate the analysis of mAbs and ADCs using multiple heart-cutting 2D-LC approach coupled with online Q-TOF MS system.

2019-03-12

(Past Events)

Dec 4, 2018 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our December 2018 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday December 4, 2018

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908)707-1545

Sponsor: Shimadzu

Shimadzu

Please  register here.  Registration is free, compliments to our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Jun Zhang

Principal Scientist, Bristol-Myers Squibb

“Evaluation of Microflow LC-MS/MS in a Quantitative Discovery Bioanalysis Setting”

Abstract for Jun Zhang:

Despite recent advances made in discovery bioanalysis to address the requirements of high throughput, high capacity and fast turnaround using LC-MS/MS, challenges in sensitivity remain for the analyses of some small molecules, peptides and other modalities. In theory microflow LC-MS/MS offers increased sensitivity compared to its regular flow counterpart due to more efficient MS ionization and sampling. In this study, the feasibility of using microflow LC-MS/MS for quantitative discovery bioanalysis was assessed. The evaluation demonstrated much improved sensitivity on microflow LC vs. regular flow LC for both small molecules and peptides, along with satisfactory analytical performance and robustness. The processes of method development, optimization and sample analysis on the microflow LC-MS/MS system were also shown to be straightforward. The better sensitivity enabled by microflow LC allowed for successful problem solving and method troubleshooting that was not feasible with regular flow LC in discovery bioanalysis.

Speaker 2:  Evelyn Wang; Ph.D.

Applications Scientist, Shimadzu Scientific Instruments

“Addressing the Challenges of Cell Culture Media Monitoring Through the Use of Triple Quad LCMS and TOF Analysis Techniques”

Abstract for Dr Wang:

Cell Culture monitoring can be challenging with all the contributing factors that can lead to cell line morbidity. As such the use of the proper tools while developing the monitoring technique is crucial to improve yields and cell line life. Once accomplished, the use of robust and reliable analysis tools to accurately map and develop intervention steps to increase the cell line growth and lifespan are crucial. This talk will discuss the merits of using the Shimadzu 9030 TOF platform for identification of components present as well as anomalies that may be observed from basal verses home grown cell line media and the use of the Shimadzu Triple Quad platform to routinely monitor cell line component consumption processes for accurate media monitoring. This process can then be transferred to manufacturing seamlessly through the use of C2MAP analysis using automated sample preparation followed by injection into the MS system for analysis.

2018-12-04

(Past Events)

Oct 9, 2018 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group The NJ Mass Spectrometry Discussion Group is pleased to announce our October 2018 Monthly Meeting. NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday, October 9, 2018

Venue:   Somerville Elks Lodge 1068

375 Union Ave Bridgewater, NJ 08807

908-707-1545

www.SomervilleElks.org

Sponsor:   Waters

Please  register here.  Registration is free, compliments of Waters.

Program

5:30 PM    Social and Registration  

6:15 PM    Complimentary Dinner

6:55 PM    Welcome and Opening Remarks

7:05 PM    Speakers

Speaker 1: Lorell Discenza

Senior Research Scientist, Bristol-Myers Squibb

“Small Molecule Bioanalysis via LC-MS/MS is Here to Stay!”

Abstract for Lorell Discenza:
Large molecules and high resolution mass spectrometers are getting all the press, but quantitative analysis of small molecules using Triple Quadrupole Mass Spectrometers in support of TK, PK and PD studies, particularly in Drug Discovery is still in high demand. To address the increasing complexity of drug targets, medicinal chemistry is taking innovative approaches in drug design, which translates into greater challenges for the Bioanalyst.

We present LC-MS/MS assay development for measuring exposures of parent compounds and their stereoisomeric metabolites as well assays for highly polar, poorly ionized compounds. And, of course, high assay sensitivity is needed in the analysis of biological matrices alcoholism such as plasma, urine, whole blood, tumor, spleen and lymph nodes.

Speaker 2:  Baiba Cabovska, Ph.D.

Senior Business Development Manager, Waters Corporation

“SHRMS and migration from food and medical product contact materials”

Abstract for Dr. Cabovska:
Extractables from packaging materials are a concern to manufacturers and suppliers of containers for the regulated food and pharmaceutical industries. The food industry is most regulated in European Union where recent regulations require testing and characterization of non-intentionally added substances in food-contact materials. Pharmaceutical industry is regulated in both the United States and Europe where extractable and leachable studies are mandatory to receive an approval for drug marketing. Due to these regulations packaging material manufacturers are motivated to control and monitor their product to ensure that no potential risk exists from extractable and leachable material.

The challenge with the compounds observed in the extraction studies is their identification. The manufacturers rarely provide a complete list of all the additives in polymers used for packaging. The original ingredients can degrade or undergo chemical changes during the manufacturing process. Also there may be contaminants that the manufacturer is not even aware of.

LetM-^Rs review how HRMS is applied for testing migration or leaching from these items to meet the regulatory requirements and to protect patients and consumers.

2018-10-09

(Past Events)

Tue Sep 18, 2018 – MSDG Vendor Show & Symposium

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our September 2018 Annual Vendor Show and Symposium.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. 

Date:  Tuesday,  September 18, 2018

Venue: Somerville Elks Lodge 1068
       375 Union Avenue
       Bridgewater, NJ 08807
       908-707-1545
       www.somervilleelks.org

Please  register here.  Registration is free, compliments of our sponsors.   [ flyer ]

A limited number of free drink tickets will be provided to early attendees!

Featuring the following sponsors / vendors / collaborators on site (listed in the order of registration):

Bruker Diagenode JEOL Eutech Instruments
Scientific Instrument Services Restek SciEx Waters
908devices IonSense Shimadzu Agilent
ThermoScientific Peak Scientific PerkinElmer Eastern Analytical Symposium

Program

3:00 – 5:00 PM        Registration, Vendor Show, and Poster Session

5:00 – 5:45 PM        Prof Hui Zhang (Johns Hopkins University)

5:45 – 7:00 PM        Buffet Dinner (raffle prizes will be announced)

7:00 – 8:00 PM        Prof Jennifer Brodbelt (University of Texas at Austin)

Prof Hui Zhang

Speaker 1: Prof Hui Zhang, PhD

Director, Mass Spectrometry Core Facility, Center for Biomarker Discovery and Translation, Department of Pathology, Johns Hopkins University

“Multi-omic analysis of tumor tissues reveals a large number of glycopeptides and their association with glycosylation enzymes”

Abstract for Prof Zhang:

Many gene products exhibit extensive structural micro-heterogeneity due to an array of co-occurring post-translational modifications. These protein modifications are not synthesized with genomic template and often affect the functionality of the proteins and therefore need to be characterized in detail in order to determine their structural and functional relationships and their potential linkage with genome and proteome. Protein glycosylation plays fundamental roles in many cellular processes, and previous reports have shown dysregulation to be associated with human diseases. Here, we describe the analysis of proteins from breast cancer xenograft tissues using our recently developed software package GPQuest 2.0, revealing a large number of previously unidentified N-linked glycopeptides. More importantly, we found that using immobilized metal affinity chromatography (IMAC) technology for the enrichment of phosphopeptides had co-enriched a substantial number of sialoglycopeptides, allowing for a large-scale analysis of sialoglycopeptides in conjunction with the analysis of phosphopeptides. Collectively, combined MS/MS analyses of proteomic and phosphoproteomic datasets resulted in the identification of thousands of intact N-linked glycopeptides derived from breast cancer xenograft tissues. This analysis revealed an extensive number of glycopeptides hidden in the proteome and co-enriched in IMAC-based phosphopeptide-enriched proteomic data.

We then performed genomic, proteomic, and glycoproteomic analysis of human high-grade serous ovarian carcinoma (HGSOC) and non-cancerous tissues, we found that abundance at glycosites was regulated by the overall glycoprotein expression, while glycosylation at each individual glycosylation site contained glycosylation-site-specific heterogeneity and it was regulated by the protein abundance of the glycoproteins as well as the levels of glycosylation enzymes that were involved in the glycosylation biosynthesis pathway. This study bridges the gaps among alterations in gene and protein expression, protein glycosylation, and phosphorylation by providing the most complete landscape of glycoproteome in related to proteome and genome, which would be beneficial for stratifying other protein modifications for changes of cancer gene products based on genetic alterations. Furthermore, using the data from glycoproteomics, proteomics, and genomics, we defined and demonstrated the possibility of classifying the pathological outcome of cancer from normal tissues of HGSOC using glycans on the glycoproteins from tissues.


Prof Jenny Brodbelt

Speaker 2: Prof Jenny Brodbelt

Norman Hackerman Chair,  Department of Chemistry, University of Texas at Austin

“Ultraviolet Photodissociation Mass Spectrometry for Characterization of Proteins and Protein Complexes”

Abstract for Prof Brodbelt:

Developments in mass spectrometry instrumentation and experimental design have motivated new applications in the field of structural biology. Ultraviolet photodissociation (UVPD) results in broad sequence coverage of intact proteins via more extensive backbone fragmentation than can be obtained using other MS/MS methods, and ion activation/dissociation can be accomplished using a single 5 ns laser pulse. This translates to a compelling MS/MS technology for characterization of intact proteins, including mapping post-translational modifications and ligand binding sites. There has been growing interest in employing top-down approaches to characterize proteins and to examine native-like protein structures by using MS/MS to disassemble the complexes and sequence the constituent proteins. UVPD provides high levels of sequence coverage for native-like protein complexes, and the relative abundances of fragment ions correlate with variations in the intramolecular and intermolecular interactions that stabilize particular regions of the proteins. Products retaining non-covalently bound ligands reflect the fast, high energy activation of UVPD.  For multimeric protein complexes, UVPD disassembles the complexes to reflect sub-unit architecture.

2018-09-18

(Past Events)

Jun 19, 2018 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group The NJ Mass Spectrometry Discussion Group is pleased to announce our June 2018 Monthly Meeting. NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday, June 19, 2018

Venue:   Somerville Elks Lodge 1068

375 Union Ave Bridgewater, NJ 08807

908-707-1545

www.SomervilleElks.org

Sponsor: Thermo Fisher

Please  register here.  Registration is free, compliments of Thermo Fisher.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Scott Peterman, PhD

Sr. Manager, Global Marketing for Advanced Products, Thermo Fisher

“Breaking the Bottlenecks of Small Molecule Research Using the Thermo Scientific Orbitrap ID-X Mass Spectrometer”

Abstract for Dr. Peterman:

Small molecule research covers a very broad range of applications, but all have similar experimental objectives for identifying and characterizing the compounds of interest to evaluate the stated research objective. Similarly, all applications face bottlenecks associated with exhaustively sampling compounds of interest in the presence of the background matrix, efficiently isolating and fragmenting each compound to generate high-quality, product ion rich spectral ion trees, and confidently automating the data processing to significantly increase the conversion of unknown unknowns to knowns. This presentation will highlight the new workflows focused on small molecules with our new tribrid mass spectrometer. The workflow components enable complex methods to be easily created, employed in automated sequence analysis routines, and demonstrations of the data processing routines used for compound identification and structural elucidation, regardless of the user expertise.

Speaker 2:  Debadeep Bhattacharyya

Sr. Manager, Marketing, Clinical Research and Forensic Toxicology, Thermo Fisher

“Confident Quantitation – Addressing Today’s and Tomorrow’s Challenges”

Abstract for Dr. Bhattacharyya:

While quantitative analysis of critical analytes in different matrices has been around for several years now, the growing complications in types of analytes and complexities of matrices continue to pose challenges to every analytical scientist focused on developing quantitative workflows. This presentation will highlight the benefits of a comprehensive workflows focused on targeted quantitation with our new generation Triple Quadrupole MS platforms. These workflows enable development of robust, reliable, reproducible, quantitative workflows for every molecule type, in every matrix regardless of the user expertise.

2018-06-19

(Past Events)

May 9, 2018 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group The NJ Mass Spectrometry Discussion Group is pleased to announce our May Monthly Meeting. NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Wednesday May 9, 2018

Venue:   Somerville Elks Lodge 1068       Note venue! 

375 Union Ave Bridgewater, NJ 08807

(908-707-1545)

Sponsor:       Bruker Daltonics

Please  register here.  Registration is free, compliments of Bruker Daltonics.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1: Prof. Leonard Foster, PhD

University of British Columbia, Vancouver, BC

“Exploring tissue-specific interactomes using PCP-SILAC”

Abstract for Dr. Foster:

An interactome describes the global organization of protein interactions within a cell. Protein correlation profiling (PCP) uses precise co-elution of two proteins as evidence that they interact. This high-throughput technique does not require overexpression or the creation of fusion proteins. We have created a SILAC mouse and used it to construct the interactomes of seven different tissues based on PCP. These data now allow us to ask several questions, including specialization of tissues at the interactome level, whether disease-causing mutations might perturb the interaction network in pathogenesis and the level of accuracy of computational predictions of interaction networks. This presentation will focus on the latest developments in interactome mapping from our laboratory.

Speaker 2:  Gary Kruppa, PhD

Vice President Proteomics, Bruker Daltonics Inc

“The timsTOF Pro Powered by PASEF: Digging Deeper into the Proteome with Record-breaking Speed, Sensitivity and Robustness”

Abstract for Dr. Kruppa:

Parallel Accumulation Serial Fragmentation (PASEF, Meier et al., JPR 2015, PMID: 26538118) for trapped ion mobility spectrometry (TIMS) quadrupole time of flight (QTOF) instruments enables 5-10X faster data-dependent acquisition of fragment ion spectra in bottom up shotgun proteomics. We have now implemented PASEF on a new instrument, the timsTOF Pro, and achieved deeper proteome coverage, as well as impressive gains in sensitivity and sample efficiency due to the 100% duty cycle and focusing of the ions in time and space provided by TIMS and the PASEF method. In this talk we will cover the principles of TIMS and PASEF and describe how these gains in speed and sensitivity are obtained. The impact of this method on instrument time and sample amount vs proteome depth in data-dependent shotgun proteomics experiments will be discussed.

2018-05-09

(Past Events)

Apr 10, 2018 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our April Monthly Meeting. NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday April 10, 2018

Venue:   Somerville Elks Lodge 1068       New venue! 

     375 Union Ave Bridgewater, NJ 08807

Sponsor: Agilent

Please  register here.  Registration is free, compliments of Agilent Technologies.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
7:00 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Dr. Jun Dai

Bristol-Myers Squibb – Discovery Analytical Sciences, Lawrenceville, NJ

“A novel online capillary isoelectric focusing – mass spectrometry (cIEF-MS) method for the separation and characterization of monoclonal antibody charge variants”

Abstract for Dr. Dai:

A reliable direct online cIEF-MS method was successfully developed, for the first time, to separate and characterize charge variants of monoclonal antibodies (mAbs). The method is based on electrokinetically pumped sheath liquid nanospray CE-MS technology using a CMP EMASS-II ion source on an Agilent TOF-MS. The cIEF-MS charge profiles are well aligned with imaged cIEF-UV results and provide valuable MS information of protein charge variants. We believe that this integral cIEF-MS platform can be a crucial technology in meeting the challenges of charge variant characterization for biotherapeutics.

Speaker 2:  Dr. Erin Baker

PNNL – Richland, WA

“Increasing Molecular Coverage in Complex Biological & Environmental Samples Using Ion Mobility-Mass Spectrometry”

Abstract for Dr. Baker:

Mass spectrometry (MS)-based technologies are playing a growing role in the analysis of complex samples. Despite significant advances in MS technology, currently it is difficult to obtain measurements of both high throughput and high sensitivity for samples with great dynamic ranges such as biofluids and plant extracts. This problem ultimately results in the inability to effectively account for variation among sample conditions and/or biodiversity leading to inconsequential findings for samples which have great variation. To address this challenge, we have coupled an ion mobility separation (IMS) with MS to afford greatly improved measurement throughput, sensitivity, robustness, and quantitative capabilities for rapid analysis of complex samples. The benefits we have observed in omic studies of biological and environmental samples with IMS-MS will be summarized in this presentation.

2018-04-10

(Past Events)

Oct 18, 2017 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our October Monthly Meeting.
NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Wednesday October 18, 2017

Venue:   Franklin Plaza Hotel   (formerly named Holiday Inn Somerset-Bridgewater)

     195 Davidson Avenue, Somerset, NJ 08873

Sponsor: Shimadzu

Shimadzu

Please  register here.  Registration is free, compliments to our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
7:00 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Prof. Richard B van Breemen

UIC/NIH Center for Botanical Dietary Supplements Research, University of Illinois College of Pharmacy, Chicago, IL; and Linus Pauling Institute, Oregon State University, Corvallis OR

“From the Field to the Clinic, the Role of Mass Spectrometry in Establishing Safety and Efficacy of Botanical Dietary Supplements”

Abstract for Prof van Breemen:

Mass spectrometry has become an essential tool for all stages of drug discovery and development. Similarly, mass spectrometry has broad utility in studies of the safety and efficacy of botanical dietary supplements. These applications to botanical dietary supplements include studies of the mechanisms of action (target identification and validation); identification of active natural products (drug discovery); studies of metabolism of active compounds; investigation of the potential for drug-botanical interactions (similar to studies of drug-drug interactions); and investigation of the pharmacokinetics of active compounds. Mass spectrometry is also being used in support of Phase I, II and III clinical trials of safety and efficacy of botanical dietary supplements. Preclinical and clinical examples will be drawn from studies of botanical dietary supplements such as red clover, black cohosh, hops, and licorice that are being used by menopausal women as alternatives to conventional hormone therapy.

Speaker 2:  Prof. Takeshi Bamba

Medical Institute of Bioregullation, Kyushu Univ., Fukoaka, Japan

“Metabolic profiling y supercritcal fluid extraction and separation technologies”

Abstract for Prof Takeshi Bamba:

Supercritical fluids have desirable properties like high density, low viscosity, and high diffusivity, which make them suitable mobile phases for supercritical fluid chromatography (SFC), an analytical technique that is amenable to high-throughput, high-resolution analysis. We attempted to apply SFC/MS to metabolic profiling. Furthermore, we tried to expand the range of applications of SFC for diverse compounds, including polar compounds. Additionally, we have developed supercritical fluid extraction (SFE) technologies for metabolic profiling. SFE can be used to extract labile compounds without degradation or loss. We applied SFE to the extraction of various metabolites and combined it with online SFC/MS for metabolic profiling. Furthermore, we developed a new online SFE-SFC-MS instrument.

2017-10-08

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