The North Jersey ACS NMR Topical Group is pleased to presents its September meeting online, Wed, Sept 23, 2020, 6:30 – 8:00 pm.
Speaker: Dr. Alexander Marchanka, Junior Professor, Institute of Organic Chemistry, Leibniz Universität, Hannover, Germany
Title: “Solid-state NMR approach for structural studies of RNA”
Large RNA molecules are challenging objects for structural biology. Solid-state NMR (ssNMR) can provide valuable structural information for large biomolecules at atomic resolution and holds great promises for structural studies of both lone RNA and RNA in complex with proteins (ribonucleoproteins, RNP). In this talk I will present our novel ssNMR methodology for structural characterization of RNA which includes sophisticated isotope labeling of RNA and acquisition of multidimensional heteronuclear correlation spectra. Additionally to conventional 13C and 15N detected ssNMR experiments we apply novel ultrafast magic angle spinning (MAS), 1H-detected ssNMR spectroscopy for structural studies of RNA. Ultrafast MAS coupled with 1H-detection extends the range of available experiments, allows deeper insights into structure and functional dynamics and significantly reduces the amount of laboratory and instrument times. Furthermore, we apply paramagnetic relaxation enhancement (PRE) to characterize protein-RNA interfaces by ssNMR. Finally, I will provide a few examples of studies on RNA, protein-RNA and RNA-RNA complexes by ssNMR and complementary techniques..
6:30pm 9/23/2020 EST (Princeton),
3:30pm 9/23/2020 PST (San Francisco),
12.30am 9/24/2020 CEST (Berlin),
7.30am 9/24/2020 JST (Tokyo)
The seminar is free of charge and sponsored by NJACS NMR Topical Group and Merck
Link to the webinar:
No registration required