Bruker Night – Dinner and speakers sponsored by Bruker
Anna Codina, PhD
Director, Pharmaceutical Business Unit, Bruker BioSpin
Donna Baldisseri, PhD
Senior Applications Scientist, Bruker BioSpin
“Validating Higher Order Structure of Biologics using NMR”
Fast 1D fingerprinting methods will give a quick answer if the biologic is similar to the reference material. To identify changes at the amino acid level, 2D NMR methods are required. An interlaboratory comparison coordinated by NIST (26 labs involved, different magnetic fields, worldwide) demonstrated both high precision and high reproducibility of the 2D methyl fingerprint NMR method. We will show recently developed and optimized acquisition techniques, including excipient signal removal. We will also introduce a newly developed software that allows an optimal workflow for batch to batch analysis of mAb samples. It provides a robust platform for the repeated application of established, tested and, recently developed data evaluation methods, namely: Profile1D (developed by Amgen) and, BiologicsHOS software (developed through the Bruker and Mestrelab strategic collaboration) which includes CCSD (Combined Chemical Shift Deviation), ECHOS (Easy Comparability of Higher Order Structure) and, PCA (Principal Component Analysis) using either the entire spectrum or peaks. We will demonstrate the workflow, and show pros and cons of the different methods.
6:00 pm Dinner (in Frick Atrium)
7:00 pm Seminar (in Seminar Room A57)
Dinner Cost (pay at the door):
$5 for everyone. No charge for seminar only.
Frick Chemistry Laboratory
Princeton, NJ 08544
Parking: Parking will be available in Lot 21 (see map link given above under Directions)
Public Transit: It is possible to take NJ Transit all the way to Princeton campus (the symposium location is ~ 10 min walk from the train station). Take the Northeast Corridor NJ transit train to Princeton Junction, then transfer to the small “dinky” train that ends on campus (5 min train ride).