North Jersey Section
American Chemical Society

Jun 19, 2019 – NMR Topical Group Meeting

The North Jersey ACS NMR Topical Group is pleased to presents its June meeting at Princeton, Wednesday, June 19, 2019 [ register ]

Bruker

Bruker Night – Dinner and speakers sponsored by Bruker

Featured Presentations

Anna Codina, PhD

Director, Pharmaceutical Business Unit, Bruker BioSpin

Anna Codina

“Bruker News”

Abstract for Anna Codina’s talk

This talk will start by highlighting Bruker’s recent innovative products and solutions. We will then focus on those applications relevant for the pharmaceutical industry, with special emphasis on the study of biologics and biosimilars by magnetic resonance spectroscopy.

Donna Baldisseri, PhD

Senior Applications Scientist, Bruker BioSpin

Donna Baldisseri

“Validating Higher Order Structure of Biologics using NMR”

Abstract for Donna Baldisseri’s talk

High-resolution nuclear magnetic resonance (NMR) is a key technology that provides critical information on protein conformation, aggregation, stability, and modifications such as glycosylation. Effectiveness and ease of use in the study of the higher order structure of proteins make this technique a uniquely valuable tool. Recently, new developments in acquisition and data analysis have emerged to establish NMR as a powerful metric for validating the HOS Critical Quality Attribute of the intact molecule utilizing both 1D and 2D NMR methods.

Fast 1D fingerprinting methods will give a quick answer if the biologic is similar to the reference material. To identify changes at the amino acid level, 2D NMR methods are required. An interlaboratory comparison coordinated by NIST (26 labs involved, different magnetic fields, worldwide) demonstrated both high precision and high reproducibility of the 2D methyl fingerprint NMR method. We will show recently developed and optimized acquisition techniques, including excipient signal removal. We will also introduce a newly developed software that allows an optimal workflow for batch to batch analysis of mAb samples. It provides a robust platform for the repeated application of established, tested and, recently developed data evaluation methods, namely: Profile1D (developed by Amgen) and, BiologicsHOS software (developed through the Bruker and Mestrelab strategic collaboration) which includes CCSD (Combined Chemical Shift Deviation), ECHOS (Easy Comparability of Higher Order Structure) and, PCA (Principal Component Analysis) using either the entire spectrum or peaks. We will demonstrate the workflow, and show pros and cons of the different methods.

Program

6:00 pm Dinner        (in Frick Atrium)
7:00 pm Seminar     (in Seminar Room A57)

Dinner Cost (pay at the door):

$5 for everyone. No charge for seminar only.

Meeting Venue

Frick Chemistry Laboratory
Princeton University
Princeton, NJ 08544 [ directions ]

Parking: Parking will be available in Lot 21 (see map link given above under Directions)

Public Transit: It is possible to take NJ Transit all the way to Princeton campus (the symposium location is ~ 10 min walk from the train station). Take the Northeast Corridor NJ transit train to Princeton Junction, then transfer to the small “dinky” train that ends on campus (5 min train ride).

Questions:

qi.gao1@merck.com (Chair) or
justyna.sikorska@merck.com (Co-Chair)

Register:

Please register online here
or via e-mail to 
qi.gao1@merck.com (Chair) or
justyna.sikorska@merck.com (Co-Chair)

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