The NJ Mass Spectrometry Discussion Group presents the
2013 Fall Vendor Show & Vendor Sponsored Workshop
Attendance is free of charge, compliments of our sponsor!
Please register here.
I. Dr. Kevin Bateman
Merck & Co
II. Professor Gary Glish
University of North Carolina at Chapel Hill
“The New Tandem MS: Ion Mobility Spectrometry/Mass Spectrometry”
- 1:30 – 7:00 PM Vendor Set Up & Registration – Hotel Ballroom
1:30 – 7:00 PM Vendor Show
3:30 – 4:15 PM Workshop Session
4:15 – 4:45 PM Cocktail Break / vendor interaction
4:45 – 5:45 PM Presentation by Dr. Kevin Bateman
5:45 – 7:00 PM Buffet Dinner (Raffle prizes will be announced)
7:00 – 8:00 PM Presentation by Professor Gary Glish
Abstract for Talk II:
Tandem mass spectrometry (MS/MS) is one of the most powerful methods available to the modern analytical chemist. However, as instruments become more and more sensitive there is an increasing challenge of isomeric and isobaric ions interfering with the analysis. The conventional approach to overcoming this problem is to use chromatography to separate analytes prior to ionization. While LC is a powerful analytical technique in its own right, it has some limitations when combined with mass spectrometry. A couple of important limitations are that the time frame of a chromatography separation is much slower than mass spectrometry (minutes to hours vs. seconds or less), and the order of analyte analysis cannot be adjusted in real-time. And for some real-time analyses, chromatography is not even an option.
As an alternative to chromatographic separations, we are developing differential ion mobility spectrometry (DIMS) as a separation method prior to mass spectrometry. While mass spectrometry separates ions based on their mass-to-charge ratio, conventional drift tube ion mobility spectrometry (DTIMS) is based on the ions’ shape-to-charge ratio. For DIMS the separation mechanism is not totally understood and while shape-to-charge plays a role, other factors such as the ion interaction with the drift gas are important. This makes DIMS more orthogonal to MS than DTIMS. DIMS has other differences compared to DTIMS that make DIMS more useful as an alternative to chromatography when combined with MS. In this presentation the fundamentals of DIMS will be discussed. Examples will be shown using DIMS/MS to analyze isobaric peptides, aerosols in real-time, and targeted compounds in complex matrices.