VENUE: Our meetings this year are at the CABM (Center for Advanced Biotechnology and Medicine) on the Rutgers Busch Campus, 679 Hoes Lane West, Piscataway NJ 08854
The meeting is in Room 010, which is located near the main entrance of CABM. Parking is available in the lot across the street from the CABM building. Dinner will be served in the meeting room.
I. “Agilent Technology Overview”
Bill Marathias Ph.D.
NMR Applications Scientist, Agilent Technologies, Inc, Boston MA
II. “NMR, Metabolomics, and Fermentation Process Analysis”
D. Christopher Roe
Corporate Center for Analytical Sciences
DuPont Experimental Station, Willmington, DE
6:00 pm Dinner
7:00 pm Presentation by Dr Marathias
7:15 pm Presentation by Dr Roe
CABM (Center for Advanced Biotechnology and Medicine) on the
Rutgers Busch Campus
- 679 Hoes Lane West, Piscataway NJ 08854
Dinner is free, compliments of our sponsor.
Abstract for Talk II:
Fermentation processes constitute a significant aspect of DuPont’s drive to sustainability and the production of renewably sourced materials. Fermentation process analysis typically involves monitoring selected metabolites and products, and although trends may be discerned, it is hard to know how to relate these observations to overall process performance. In an effort to accelerate fermentation process development, multivariate methods are being applied to combined fermentation and analytical data sets in order to provide an overview of the fermentation process. The goal is to identify differentially expressed metabolites that contribute significantly to the differences between fermentations (e.g., growth variability, high vs. low titer, or one strain vs. another). This information can be assessed for biological significance, and metabolic engineering can be considered for the identified metabolic pathways. The merits of NMR for metabolomic analysis will be described along with data “pre-processing” methods prior to multivariate analysis. A synopsis of multivariate methods will be given and examples of fermentation time course studies will be presented.