The NJ Mass Spectrometry Discussion Group presents its May Meeting on Tuesday, May 12, 2015 at at the Holiday Inn Somerset-Bridgewater, 195 Davidson Ave, Somerset NJ 08873
Sponsored by Waters
The winners of the student ASMS travel awards will be announced during the meeting.
Please register here.
5:30 pm Social and registration
6:15 pm Complimentary dinner
7:00 pm Welcome and opening remarks
7:05 pm Speakers
(1) Bernard Choi Ph.D, Principal Scientist, Merck & Co., Inc., Rahway, NJ
“Automation of sample preparation for LC-MSMS analysis of small molecules and peptides in biological”
(2) David McLaren Ph.D, Principal Scientist, Merck & Co., Inc., Kenilworth, NJ
“TApproaches to Biomarker Discovery for Lipid Metabolizing Enzymes in Pharmaceutical Research & Development”
7:55 pm Closing remarks
Abstract (1): The presentation will discuss different approaches to implementing automated sample preparation for LC-MSMS (i.e. protein precipitation, solid phase extraction and immunoprecipitation).
Abstract (2): Disorders of lipid metabolism are contributing causes or comorbidities in a variety of diseases including hyperlipidemia, pancreatitis, type 2 diabetes and atherosclerosis. Discovery and development of novel therapies to treat these disorders depends on reliable methods to measure effects on lipid homeostasis in vivo. To this end, we have developed fit-for-purpose general protocols for studying modulation of lipid metabolizing enzymes in preclinical species specifically to enable rapid evaluation of pharmacodynamic effects. This presentation will describe examples of biomarker development in the area of dyslipidemia which can be used to support research in the fields of cardiovascular disease and diabetes. The use of key enabling technologies, including lipidomics and metabolic flux using stable isotope tracers, will be highlighted. Particular emphasis will be given to experimental considerations impacting on analysis and interpretation of the stable isotope tracer data. Finally, the impact that higher sensitivity mass spectrometers can have on pushing the limits of metabolic flux studies will be discussed.