North Jersey Section
American Chemical Society

Oct 9, 2019 – MSDG Monthly Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our October 2019 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Wednesday October 9, 2019

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908)707-1545

Sponsor: Bruker

Bruker Daltonics

 
Please  register here.  Registration is free, compliments of our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Prof. Peter Jackson, Ph.D.

Stanford University

“Cancer Mechanisms and Profiling from Analysis of AP/MS and Shotgun Proteomics”

Abstract for Peter Jackson:

One fifth of human cancers contain mutations activating the Ras GTPases, but no targeted therapies are yet available. Many effectors of the Ras pathway and potential targets remain underexplored and we have limited understanding of numerous paralogs of Ras effectors and other redundancies that contribute to Ras tumor growth. Here, we produce a multiomic map of the Ras function in lung adenocarcinoma. We use affinity purification mass spectrometry (AP/MS) to generate a protein-protein interaction (PPI) network. From this network, we constructed a sgRNA library covering 120 Ras pathway genes and screened for genetic interactions (GIs) by CRISPR dual knockout. We find new effectors of Ras-driven cellular function, and over 250 synthetic lethal GIs, presenting new candidate single and combination therapeutic targets. We identify important synergies between Ras and Rap signaling and between their downstream effector pathways. Our recent studies have used selective covalent inhibitors of a KRAS-G12C allele from the Shokat lab to address global signaling changes by shotgun proteomics, using the Bruker timsTOF. I will present the latest technical details for our data and also outline our informatics pipeline, which provides critical validation of our MS findings. These discoveries illustrate the power of multiomic interaction-mapping approach and may be applied to analyze a range of human diseases or other biological systems.

Speaker 2:  Tharan Srikumar, Ph.D.

Bruker Daltonics

“New Dimensions in Confidence for OMICS and HCPs with TIMS Technology”

Abstract for Tharan Srikumar:

Confidence in mass spectrometry measurements is key for advancing drug discovery projects. Bruker provides a comprehensive line of ESI and MALDI TOF instruments as well industry leading ultrahigh resolution MRMS systems and the evolutionary timsTOF Pro. The timsTOF Pro with PASEF relies on a unique dual Trapped Ion Mobility Spectrometry (TIMS) front end to provide nearly 100% duty cycle with no loss of ions. When combined with the Parallel Accumulation Serial Fragmentation (PASEF) method, the timsTOF Pro with PASEF enables sequencing speeds in excess of 100 Hz, and significant improvements in sensitivity. Powered by TIMS, the instrument provides separation for simple isomers and conformers and stands as an integrated part of 4D omics workflows because of the accurate and reproducible collisional cross-sections (CCS) values for all peptides. Recently, the TIMS technology has been applied to host cell protein (HCP) analysis of mAbs with record speed and depth. Newly announced diaPASEF enables more ways to accurately quantify proteins. The presentation will show how the technology can benefit HCP analysis and OMICS to step up the research to the next level.

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