North Jersey Section
American Chemical Society

Dec 4, 2018 – MSDG Meeting

NJ-ACS Mass Spec Discussion Group
The NJ Mass Spectrometry Discussion Group is pleased to announce our December 2018 Monthly Meeting.

NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]

Date:    Tuesday December 4, 2018

Venue:   Somerville Elks Lodge 1068

     375 Union Avenue, Bridgewater, NJ 08807

     (908)707-1545

Sponsor: Shimadzu

Shimadzu

Please  register here.  Registration is free, compliments to our sponsor.

Program

5:30 PM    Social and Registration  
6:15 PM    Complimentary Dinner
6:55 PM    Welcome and Opening Remarks
7:05 PM    Speakers

Speaker 1:  Jun Zhang

Principal Scientist, Bristol-Myers Squibb

“Evaluation of Microflow LC-MS/MS in a Quantitative Discovery Bioanalysis Setting”

Abstract for Jun Zhang:

Despite recent advances made in discovery bioanalysis to address the requirements of high throughput, high capacity and fast turnaround using LC-MS/MS, challenges in sensitivity remain for the analyses of some small molecules, peptides and other modalities. In theory microflow LC-MS/MS offers increased sensitivity compared to its regular flow counterpart due to more efficient MS ionization and sampling. In this study, the feasibility of using microflow LC-MS/MS for quantitative discovery bioanalysis was assessed. The evaluation demonstrated much improved sensitivity on microflow LC vs. regular flow LC for both small molecules and peptides, along with satisfactory analytical performance and robustness. The processes of method development, optimization and sample analysis on the microflow LC-MS/MS system were also shown to be straightforward. The better sensitivity enabled by microflow LC allowed for successful problem solving and method troubleshooting that was not feasible with regular flow LC in discovery bioanalysis.

Speaker 2:  Evelyn Wang; Ph.D.

Applications Scientist, Shimadzu Scientific Instruments

“Addressing the Challenges of Cell Culture Media Monitoring Through the Use of Triple Quad LCMS and TOF Analysis Techniques”

Abstract for Dr Wang:

Cell Culture monitoring can be challenging with all the contributing factors that can lead to cell line morbidity. As such the use of the proper tools while developing the monitoring technique is crucial to improve yields and cell line life. Once accomplished, the use of robust and reliable analysis tools to accurately map and develop intervention steps to increase the cell line growth and lifespan are crucial. This talk will discuss the merits of using the Shimadzu 9030 TOF platform for identification of components present as well as anomalies that may be observed from basal verses home grown cell line media and the use of the Shimadzu Triple Quad platform to routinely monitor cell line component consumption processes for accurate media monitoring. This process can then be transferred to manufacturing seamlessly through the use of C2MAP analysis using automated sample preparation followed by injection into the MS system for analysis.

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