The NJ Mass Spectrometry Discussion Group is pleased to announce our May Monthly Meeting. NJ MSDG is the second largest mass spectrometry professional association in the nation behind ASMS, with over 1,100 members in the tristate area. [ homepage ]
Date: Wednesday May 9, 2018
Venue: Somerville Elks Lodge 1068 Note venue!
375 Union Ave Bridgewater, NJ 08807
Please register here. Registration is free, compliments of Bruker Daltonics.
5:30 PM Social and Registration
6:15 PM Complimentary Dinner
6:55 PM Welcome and Opening Remarks
7:05 PM Speakers
Speaker 1: Prof. Leonard Foster, PhD
University of British Columbia, Vancouver, BC
“Exploring tissue-specific interactomes using PCP-SILAC”
Abstract for Dr. Foster:
An interactome describes the global organization of protein interactions within a cell. Protein correlation profiling (PCP) uses precise co-elution of two proteins as evidence that they interact. This high-throughput technique does not require overexpression or the creation of fusion proteins. We have created a SILAC mouse and used it to construct the interactomes of seven different tissues based on PCP. These data now allow us to ask several questions, including specialization of tissues at the interactome level, whether disease-causing mutations might perturb the interaction network in pathogenesis and the level of accuracy of computational predictions of interaction networks. This presentation will focus on the latest developments in interactome mapping from our laboratory.
Speaker 2: Gary Kruppa, PhD
Vice President Proteomics, Bruker Daltonics Inc
“The timsTOF Pro Powered by PASEF: Digging Deeper into the Proteome with Record-breaking Speed, Sensitivity and Robustness”
Abstract for Dr. Kruppa:
Parallel Accumulation Serial Fragmentation (PASEF, Meier et al., JPR 2015, PMID: 26538118) for trapped ion mobility spectrometry (TIMS) quadrupole time of flight (QTOF) instruments enables 5-10X faster data-dependent acquisition of fragment ion spectra in bottom up shotgun proteomics. We have now implemented PASEF on a new instrument, the timsTOF Pro, and achieved deeper proteome coverage, as well as impressive gains in sensitivity and sample efficiency due to the 100% duty cycle and focusing of the ions in time and space provided by TIMS and the PASEF method. In this talk we will cover the principles of TIMS and PASEF and describe how these gains in speed and sensitivity are obtained. The impact of this method on instrument time and sample amount vs proteome depth in data-dependent shotgun proteomics experiments will be discussed.