Speaker: Silvia Cavagnero
Position: Professor
Organization: University of Wisconsin-Madison, Madison

Date and Time: Thursday June 5th, 2025 @ 12pm EDT on MS teams

Title: LC-Photo-CIDNP: a gateway to hypersensitive NMR spectroscopy

Abstract :Liquid-state low-concentration photochemically induced dynamic nuclear polarization (LC-photo-CIDNP) is an emerging technology capable of enhancing the sensitivity of NMR spectroscopy via LED-mediated optical irradiation. LC-photo-CIDNP enables the detection of solvent-exposed aromatic residues in isolation and within polypeptides and proteins. This presentation will focus on describing how unlabeled small molecules and amino-acid isotopologs bearing quasi-isolated spin pairs (QISP) either as free species or within proteins lead to un-precedented nuclear-spin hyperpolarization due to both dark (LED-off) and light (LED-on) effects. The magnetic-field dependence of LC-photo-CIDNP of biomolecule (within the high-field regime) is particularly interesting, and will be described in the context of low-field NMR and field cycling. Large enhancement factors (e.g., 1,200) were obtained at 50 MHz (1.18 T), suggesting exciting avenues to explore the early and late stages of protein folding and aggregation in physiologically relevant environments at high sensitivity.

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Meeting ID: 272 352 880 226
Passcode: KT7NK7aT