The North Jersey ACS NMR
Topical Group Proudly Presents:
The May Meeting
Wednesday
May 21st,
2008
Dr. Steve Cheatham
NMR
Spectroscopist
DuPont , Wilmington, Delaware
“19F-X Correlation Experiments for Heterocyclic Analysis”
Abstract:
The frequent inclusion of fluorine in bioactive compounds presents both challenge and opportunity for structural analysis. 19F-15N long range correlations in particular are extremely valuable in deducing structure in small heterocyclic systems. We have examined a variety of heterocyclic types and the practical aspects of interpreting long range correlation data from 19F will be discussed. In addition, the challenges of performing experiments on samples where spectral dispersion exceeds the typical 90 degree excitation bandwidth of the probe will be examined. Traditionally, large proteins, unstable proteins, and aggregation-prone proteins have been difficult to examine by NMR spectroscopy. The reverse micelle approach to NMR can allow proteins in each of these classes to be examined, by encapsulating the protein within the shell of a reverse micelle, and dissolving this reverse micelle in a low viscosity fluid.
Here we
illustrate the application of the reverse micelle approach to NMR to several
interesting problems: NMR of large soluble proteins; the forced folding of an
unstructured protein in the confined space of a reverse micelle, and the
solubilization of a membrane protein. To achieve these advances we have greatly
expanded the library of reverse micelle surfactants that have been determined
to support protein encapsulation at sufficient concentrations; developed and
appropriate encapsulation environment for the KcsA
membrane protein, determined structural constraints from reverse micelle
alignment, redesigned the apparatus required to prepare and maintain reverse
micelle samples; and have achieved the routine use of liquid ethane, an
extremely low viscosity fluid.
Door Prizes!
Place: Woodbridge
Hilton, Iselin, NJ
(Directions: http://njacs.org/d_woodhilt.html)
Dinner at 6:30
p.m.
Seminar at 7:00 p.m.
Dinner
cost: $30 ($5 for student/postdoc)
No charge for seminar only.
Register online: below
or via e-mail to:
