Abstract:  Mass-spectrometry has the ability to elucidate new diagnostic, prognostic, and therapeutic biomarkers and translate them to the clinic. However, there are numerous challenges that exist that must be overcome. This presentation will discuss the challenges specific to the study of epithelial ovarian cancer (EOC) in humans and how these challenges have directed our thinking in terms of the development of mass spectrometry-based bioanalytical strategies. First, to augment the human model, we have developed a chicken model of spontaneous EOC which allows us to control the environment and genetic background, a model which is characterized by rapid onset and progression of disease, and allows us to conduct longitudinal sampling. Second, we have developed hydrophobic tagging reagents to increase the electrospray response of N-linked glycans which has the major added benefit of being able to incorporate a stable-isotope label for use in relative quantification experiments. Finally, we have developed new ionization methods that can be used for direct analysis and tissue imaging; the latter being a fruitful arena for biomarker discovery.

Biography:  David C. Muddiman is currently a Professor of Chemistry and Founder and Director of the W.M. Keck FT-ICR Mass Spectrometry Laboratory at North Carolina State University in Raleigh, NC. Prior to moving his research group to North Carolina State University, David was a Professor of Biochemistry and Molecular Biology and Director of the Mayo Proteomics Research Center at the Mayo Clinic College of Medicine in Rochester, MN. Prior to his appointment at the Mayo Clinic, David was an Associate Professor of Chemistry at Virginia Commonwealth University where he began his academic career as an assistant professor in 1997 with an adjunct appointment in the Department of Biochemistry & Molecular Biophysics where he was also a member of the Massey Cancer Center. David was born in Long Beach, CA in 1967 but spent most of his formative years in a small town in Pennsylvania. David received his B.S. in chemistry from Gannon University (Erie, PA) in 1990 and his Ph.D. in Analytical Chemistry from the University of Pittsburgh in 1995 under the auspices of David M. Hercules. He then was a Department of Energy Postdoctoral Fellow at Pacific Northwest National Laboratory in the Environmental Molecular Sciences Laboratory working with Richard D. Smith from 1995-1997. David has served on over 35 NIH study sections since 1999 and reviews for over 30 scientific journals. He has been a guest editor for Analytical and Bioanalytical Chemistry, Mass Spectrometry Reviews, the Journal of the American Society for Mass Spectrometry and the Internal Journal of Mass Spectrometry. He currently serves on the Editorial Advisory Board of Mass Spectrometry Reviews, Journal of Proteome Research, Rapid Communications in Mass Spectrometry, Journal of the American Society for Mass Spectrometry, Advances in Physical Chemistry, and the Journal of Chromatography B. He also serves on the advisory board of the National Science Foundation FT-ICR Mass Spectrometry Laboratory, National High Magnetic Field Laboratory, Florida State University and the Yale/NIDA Neuroproteomics Center, Yale University. His group has presented over 350 invited lectures and presentations at national and international meetings, has published over 160 peer-reviewed papers, and has received three US patents. He is the recipient of the 2010 Biemann Medal, American Society for Mass Spectrometry, 2009 NCSU Alumni Outstanding Research Award, the 2004 ACS Arthur F. Findeis Award, the 1999 American Society for Mass Spectrometry Research Award, and the Safford Award, University of Pittsburgh, for Excellence in Teaching. [ Visit Dr. Muddiman's Website ]