North Jersey Section
American Chemical Society

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Jun 18, 2013 – MSDG Meeting

NJ-ACS Mass Spec Discussion GroupThe NJ Mass Spectrometry Discussion Group presents the June 2013 Meeting on Tuesday, June 18, 2013 at Mirabelle’s Room, DoubleTree by Hilton Somerset, 200 Atrium Drive, Somerset, New Jersey 08873.

Sponsored by Thermo.

Thermo Scientific

Attendance is free of charge, compliments of our sponsor!

Please register here.

Featured Speakers

I. Jonathan Josephs, Ph.D

Principal Scientist, Bristol-Myers Squibb

“Creation of Rich MSn Data for Structure Elucidation in Quan/Qual Analysis: Applications of Combined HCD/CID and New Isolation Techniques”

II. Dr. Jie Qian

“Orbitrap Analysis of Native Intact Monoclonal Antibody”

Program

    5:30 pm Social and registration
    6:15 pm Complimentary dinner
    7:00 pm Welcome and opening remarks
    7:05 pm Jonathan Josephs
    8:00 pm Jie Qian
    8:55 pm Closing remarks

Abstract for Talk II:

    Introduction — Because monoclonal antibodies (mAbs) are increasingly an important line of therapeutics for the biopharmaceutical industry, the demand to better understand the biochemical and biophysical properties of mAbs has become critical. Recent developments in native protein mass spectrometry (MS) have clearly shown its distinctive power for characterization of intact proteins and protein conjugates. The mass spectrometry–based study of intact mAbs in native-like states provides a wealth of information to interpret its biological features. This is the first reported use of a commercial Orbitrap mass analyzer for measuring a native intact mAb protein. A monoclonal IgG1 kappa antibody, composed of two heavy chains of 451 amino acids and two light chains of 213 amino acids was characterized in this study.

    Methods — The mAb was analyzed using normal flow liquid chromatography (LC)-MS method. The intact mAb protein was eluted from a size exclusion column under non-denaturing and non-reducing conditions and then directly detected by a standard stock bench-top Orbitrap mass spectrometer under full scan MS mode. The AGC target was set at 3e6 with resolution at 17,500. The MS spectrum was then analyzed with protein deconvolution software (Protein Deconvolution 2.0) that utilizes the ReSpect algorithm for molecular mass determination.

    Results — To monitor the IgG1 kappa in native-like state, the protein was dissolved under non-denatured and non-reduced conditions. The protein was then chromatographically eluted from a size exclusion column and directly analyzed online using an Orbitrap mass spectrometer with high mass range. The mass spectra from LC-MS analyses of the native intact antibody demonstrate a clean distribution of the multiply charged envelope at mass range above 5000 m/z. Individual charge states in the raw mass spectrum for the native antibody shows major glycosylation species. Results from this study demonstrate an efficient and potentially high through-put workflow for antibody measurement in native-like states. This protocol, using a combination of normal flow size exclusion LC and a HR/AM bench-top Orbitrap mass spectrometer is expected to provide important data for mAb studies.

    Novel Aspect — Native Intact Monoclonal Antibody was analyzed by a standard stock bench-top Orbitrap mass spectrometer

2013-06-18

(Past Event)

May 7, 2013 – MSDG Meeting

NJ-ACS Mass Spec Discussion GroupThe NJ Mass Spectrometry Discussion Group presents the May 2013 Meeting on Tuesday, May 7, 2013 at the Holiday Inn Somerset-Bridgewater, 195 Davidson Ave, Somerset NJ 08873. Sponsored by Agilent Technologies.

Attendance is free of charge, compliments of our sponsors!

Please register here.

Featured Speakers

I. Xinping Fang, Ph.D

VP, Head of Bioanalytical & Acting Head of Drug Metabolism / Biotransformation, XenoBiotic Laboratories, Inc, Plainsboro, NJ.

“Implementing an Ultrasensitive and Advanced New LC-MS/MS Platform, Xevo TQ-S, in a Regulated BA Lab”

II. Paul Rainville

Senior Manager of Scientific Operations, ESD, Pharmaceutical & Life Sciences Business Operations. Waters Corporation

“Advances in Front-End Technologies for Bioanalysis”

Program

    5:30 pm Social and registration
    6:15 pm Complimentary dinner
    7:00 pm Welcome and opening remarks
    7:05 pm Xinping Fang
    8:00 pm Paul Rainville
    8:55 pm Closing remarks

Abstract for Talk II:

Modern bioanalytical chemists are charged with the task of developing high sensitivity assays for drug candidates with low circulating concentration, typically in the single pg/mL range. If this challenge was not difficult enough new economic, study design and ethical pressures demand that increasingly small volumes of samples are required and new sample formats such as dried blood spots. In this paper we will discuss the relative merits and pitfalls of two-dimensional liquid chromatography for bioanalytical applications when coupled to tandem quadrupole MS. Two-dimensional chromatography was evaluated for two applications i) to improve analyte cleanliness, thus increasing assay sensitivity and ii) to allow the direct injection of organic solvents onto a reversed phase system. As part of the study the mode of 2D operation was evaluated; forward flush, back flush and heart cut. Also we will discuss the use of a micro fabricated ceramic device packed with sub 2ìm for the analysis of plasma, bile and urine samples. We will show how the use of the micro scale separations device has been employed for the analysis of both small and large molecules.

2013-05-07

(Past Event)

Apr 9, 2013 – MSDG Meeting

The NJ Mass Spectrometry Discussion Group presents the April 2013 Meeting on Tuesday, April 9, 2013 at the Holiday Inn Somerset-Bridgewater, 195 Davidson Ave, Somerset NJ 08873. Sponsored by Agilent Technologies.

Attendance is free of charge, compliments of our sponsors!

Please register here.

Featured Speakers

Alexander Maggitti

    National Medical Services, Inc.

“Accurate Mass Screening of Biological Matrices via Liquid Chromatography Time of Flight Mass Spectrometry: Techniques, Applications, & Results in the Forensic Laboratory”

Dr. Eric Milgram

    Director, Metabolomics and Nutrient Physiology,
    PepsiCo Biology Innovation Laboratory based in New Haven, CT

Program

    5:30 pm Social and registration
    6:15 pm Complimentary dinner
    7:00 pm Welcome and opening remarks
    7:05 pm Alexander Maggitti
    8:00 pm Eric Milgram
    8:55 pm Closing remarks

2013-04-09

(Past Event)

Mar 12, 2013 – MSDG Meeting

The NJ Mass Spectrometry Discussion Group presents the March 2013 Meeting on Tuesday, March 12, 2013 at the Holiday Inn Somerset-Bridgewater, 195 Davidson Ave, Somerset NJ 08873. Sponsored by Bruker & ImaBiotech SA.

Attendance is free of charge, compliments of our sponsors!

Please register here.

Featured Speakers

Rohan Thakur
Vice President, Chemical and Applied Markets, Bruker
“Peptide Quantitation at Analytical Flow Rates – Challenges with Ionization at High-flow Rates”

 

Jonathan Stauber
CEO / CSO ImaBiotech, ImaBiotech SA
“Label-free MS imaging from drug discovery to preclinical development


Rohan Thakur

Jonathan Stauber

Program

    5:30 pm Social and registration
    6:15 pm Complimentary dinner
    7:00 pm Welcome and opening remarks
    7:05 pm Rohan Thakur
    8:00 pm Jonathan Stauber
    8:55 pm Closing remarks

Abstract for Jonathan Stauber: Mass Spectrometry Imaging (MSI) has become a common technique to localize molecules directly on the surface of biological tissues without labeling. Recently, ImaBiotech performed quantitative studies by developing and using dedicated software (Quantinetix) and combined the quantitative MS imaging with Metabolomics studies with High Resolution Mass Spectrometry Imaging. This presentation shows the benefits of MS imaging applications in different therapeutic areas (CNS, Oncology, Ophthalmology) from discovery to preclinical development stages.

Abstract for Rohan Thakur: Targeted quantification of peptides is now becoming routine. The challenges with micro-flow/nano-flow work are well understood and fall short when compared to the method robustness demonstrated by small molecule analysis over the past 20 years. To get the required robustness for peptide analysis, one can use higher flow rates with UHPLC and traditional heated electrospray settings – the issue then becomes ensuring optimal ionization and avoiding thermal breakdown before the ionization step in modern high flow heated electrospray methods. This presentation will show the use of cutting-edge material science technology to provide optimal ionization of peptides at analytical flow rates, especially thermally labile components.

2013-03-12

(Past Event)

Dec 6, 2012 – MSDG Meeting – AB Sciex Night

The NJ Mass Spectrometry Discussion Group presents the December Meeting – AB Sciex Night at the Holiday Inn Somerset-Bridgewater, 195 Davidson Ave, Somerset NJ 08873 on Thursday, December 6, 2012. Sponsored by AB Sciex.

  • Challenges and Solutions in Large Molecule Regulated Bioanalysis using High Resolution Mass Spectrometry”, Dr. Fabio Garofolo, Vice President Bioanalytical Services, Algorithme Pharma
  • Micro Flow Liquid Chromatography MS/MS: The Present and Future in Bioanalytical Laboratories”, Chad Christianson, Casey Johnson, Jennifer Zimmer and Dr. Shane Needham (Presenter), Laboratory Director Alturas Analytics, Inc.

5:30 pm Social and registration
6:15 pm Complimentary dinner
7:00 pm Welcome and opening remarks
7:05 pm Dr. Fabio Garofolo, Bioanalytical Services, Algorithme Pharma
8:00 pm Dr. Shane Needham, Alturas Analytics, Inc.
8:55 pm Closing remarks

Attendance is free of charge, compliments of our sponsor!

Back: Ron Kong, Donna duPont, Fangbiao Li, Robert Iannucci, Jonathan Ho; Front: Brett Peterson, Fabio Garofolo, Shane Needham, Graham Gibson.
Back: Ron Kong, Donna duPont, Fangbiao Li, Robert Iannucci, Jonathan Ho; Front: Brett Peterson, Fabio Garofolo, Shane Needham, Graham Gibson.
Dr. Fabio Garofolo,Vice President Bioanalytical Services, Algorithme Pharma, presented in NJ MSDG 2012 December seminar.
Dr. Fabio Garofolo,Vice President Bioanalytical Services, Algorithme Pharma, presented in NJ MSDG 2012 December seminar.
Dr. Shane Needham,Laboratory Director Alturas Analytics, Inc., presented in NJ MSDG 2012 December seminar.
Dr. Shane Needham,Laboratory Director Alturas Analytics, Inc., presented in NJ MSDG 2012 December seminar.

2012-12-06

Photos From 2012 Events


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